Immortalized cell lines derived from dental/odontogenic tissue.
Yanglin Zeng1, Liu Liu1, Dingming Huang1,2
1State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, National Center for Stomatology, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.
Cell and Tissue Research
|April 11, 2023
Summary
Dental stem cells offer regenerative potential but face limited proliferation due to senescence. Immortalization strategies, using genes like hTERT, overcome this, enabling unlimited growth for dental regenerative medicine.
Area of Science:
- Biomedical Engineering
- Stem Cell Biology
- Regenerative Medicine
Background:
- Dental/odontogenic stem cells possess multipotent differentiation capabilities, crucial for tooth regeneration and research.
- In vitro studies are limited by rapid cellular senescence and restricted proliferation of these valuable cells.
- Cellular immortalization presents a viable solution to overcome proliferation limitations.
Purpose of the Study:
- To review the establishment and applications of immortalized stem cells from dental/odontogenic tissues.
- To discuss the advantages and challenges associated with immortalizing these specific cell types.
- To highlight the potential of immortalized dental stem cells in regenerative medicine.
Main Methods:
- Introduction of oncogenes, human telomerase reverse transcriptase (hTERT), or other immortalization genes.
- Generation of immortalized cell lines from various dental sources, including dental pulp stem cells (DPSCs), periodontal ligament cells (PDLs), stem cells from human exfoliated deciduous teeth (SHEDs), dental papilla cells (DPCs), and tooth germ mesenchymal cells (TGMCs).
Main Results:
- Successful generation of diverse immortalized dental stem cell lines.
- Demonstration of unlimited proliferation capacity in these immortalized cells.
- Established protocols for generating and utilizing immortalized dental stem cells.
Conclusions:
- Immortalization overcomes senescence, unlocking the full potential of dental stem cells for research and therapy.
- Immortalized dental stem cells provide a robust platform for advancing tooth regenerative medicine.
- Further research is needed to fully address the challenges and optimize the applications of immortalized dental stem cells.
Keywords:
ApplicationCellular senescenceImmortalizationStem cells from dental/odontogenic tissuesStrategiesMore Related Videos
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