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Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
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Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
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Somatic Cell Nuclear Transfer in Pigs.

Werner G Glanzner1, Vitor B Rissi2, Vilceu Bordignon3

  • 1Department of Animal Science, McGill University, Sainte-Anne-de-Bellevue, QC, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|April 11, 2023
PubMed
Summary

Somatic cell nuclear transfer (SCNT) successfully clones pigs, a vital livestock and biomedical model. This chapter details a protocol for producing cloned pigs using SCNT, advancing agricultural and research applications.

Keywords:
Embryo transferEnucleationHistone deacetylasesNuclear reprogrammingNuclear transplantationSCNTSus scrofaSwineTranscriptional inhibition

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Area of Science:

  • Animal cloning
  • Reproductive biology
  • Biotechnology

Background:

  • Somatic cell nuclear transfer (SCNT) is a key technique for animal cloning.
  • Pigs are significant livestock for food and biomedical models due to human physiological similarities.
  • SCNT has been used to produce pig clones for agricultural and research purposes over the last two decades.

Purpose of the Study:

  • To present a detailed protocol for producing cloned pigs via SCNT.
  • To facilitate the application of SCNT technology in swine for various uses.

Main Methods:

  • The chapter outlines a specific protocol for somatic cell nuclear transfer in pigs.
  • Key steps and considerations for successful SCNT in swine are described.

Main Results:

  • The described SCNT protocol enables the production of cloned pigs.
  • This method supports the generation of genetically identical swine for diverse applications.

Conclusions:

  • SCNT provides a viable method for pig cloning.
  • The protocol detailed in this chapter can be utilized for both agricultural and biomedical swine cloning efforts.