Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Reproductive Cloning01:27

Reproductive Cloning

30.6K
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic...
30.6K
Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

2.0K
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
2.0K
Cloning of Dolly the Sheep01:08

Cloning of Dolly the Sheep

4.1K
The first successfully cloned mammal was Dolly, a sheep, born on 5th July 1996 at Roslin Institute, Scotland. The cloned sheep was named after the American singer Dolly Parton. Dolly lived for seven years and died of respiratory complications, which is speculated to be due to the actual age of her DNA. Because the DNA in cloned cells belongs to an older individual,  the cloned individual’s life expectancy may be affected. Indeed, analysis of Dolly’s DNA revealed shorter...
4.1K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

1.9K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.9K
Plant Tissue Culture02:57

Plant Tissue Culture

38.1K
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
38.1K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.3K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In Vitro Sperm-Epididymosomes Interaction Immediately Before Fertilization Changes Sperm Fertility Potential.

Andrology·2026
Same author

Effect of Small Molecules on Blastocyst Development and Outgrowth Establishment of Bovine Haploid Parthenogenetic Embryos.

Animals : an open access journal from MDPI·2026
Same author

CD30 Expression in Neoplastic Mast Cells and the Presence of CD30+, CD3+, and PAX-5+ Tumour-Infiltrating Lymphocytes as Prognostic Markers in Canine Cutaneous Mast Cell Tumours.

Veterinary and comparative oncology·2026
Same author

Effects of Liposome-Encapsulated α-Pinene on In Vitro Oocyte Maturation and Embryo Development in Bovine Species.

Molecular reproduction and development·2026
Same author

Follicular fluid extracellular vesicles improve bovine oocyte quality via lipid and mitochondrial modulation.

Frontiers in veterinary science·2026
Same author

LOX and LOXL2 Expression in Canine Mammary Carcinomas.

Veterinary and comparative oncology·2025
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Aug 3, 2025

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer
09:52

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer

Published on: April 26, 2018

12.7K

Cattle Cloning by Somatic Cell Nuclear Transfer.

Juliano Rodrigues Sangalli1, Rafael Vilar Sampaio1,2, Tiago Henrique Camara De Bem1

  • 1Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brazil.

Methods in Molecular Biology (Clifton, N.J.)
|April 11, 2023
PubMed
Summary
This summary is machine-generated.

Somatic cell nuclear transfer (SCNT) cloning faces efficiency challenges due to epigenetic errors. This chapter details a bovine SCNT protocol to produce live cloned calves and improve nuclear reprogramming for biotechnological applications.

Keywords:
CattleNuclear transplantationReprogramming

More Related Videos

Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte
06:15

Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte

Published on: July 6, 2022

2.2K
Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT
13:36

Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT

Published on: September 30, 2010

14.2K

Related Experiment Videos

Last Updated: Aug 3, 2025

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer
09:52

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer

Published on: April 26, 2018

12.7K
Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte
06:15

Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte

Published on: July 6, 2022

2.2K
Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT
13:36

Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT

Published on: September 30, 2010

14.2K

Area of Science:

  • Reproductive biology
  • Epigenetics
  • Biotechnology

Background:

  • Somatic cell nuclear transfer (SCNT) can reprogram differentiated cells to totipotency.
  • SCNT is valuable for generating animals, stem cells, and applications in cell therapy and drug screening.
  • Low efficiency and high cost limit widespread SCNT adoption, primarily due to epigenetic reprogramming errors.

Purpose of the Study:

  • To discuss epigenetic constraints limiting SCNT efficiency.
  • To present a bovine SCNT protocol for producing live cloned calves.
  • To provide a foundation for improving SCNT techniques.

Main Methods:

  • Review of epigenetic barriers in SCNT.
  • Detailed description of a bovine SCNT protocol.
  • Discussion of strategies to correct epigenetic errors.

Main Results:

  • A reproducible bovine SCNT protocol yielding live cloned calves was established.
  • Key aspects of nuclear reprogramming were addressed.
  • The protocol serves as a basis for future SCNT improvements.

Conclusions:

  • Overcoming epigenetic errors is crucial for enhancing SCNT efficiency.
  • The presented bovine SCNT protocol offers a practical approach for research.
  • Further research integrating epigenetic correction strategies can advance SCNT technology.