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Published on: September 30, 2010
Derivation of Bovine Primed Embryonic Stem Cells from Somatic Cell Nuclear Transfer Embryos
Delia A Soto1, Micaela Navarro1,2, Pablo J Ross3
1Department of Animal Science, University of California, Davis, CA, USA.
Abstract:
Derivation of bovine embryonic stem cells from somatic cell nuclear transfer embryos enables the derivation of genetically matched pluripotent stem cell lines to valuable and well-characterized animals. In this chapter, we describe a step-by-step procedure for deriving bovine embryonic stem cells from whole blastocysts produced by somatic cell nuclear transfer. This simple method requires minimal manipulation of blastocyst-stage embryos, relies on commercially available reagents, supports trypsin passaging, and allows the generation of stable primed pluripotent stem cell lines in 3-4 weeks.
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