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Updated: Aug 3, 2025
A Multi-Omics Extraction Method for the In-Depth Analysis of Synchronized Cultures of the Green Alga Chlamydomonas reinhardtii
Published on: August 8, 2019
Ultrasound-induced cell disintegration and its ultrastructure characterization for the valorisation of Chlorella
Yong Deng1, Xiaoling Yang1, Tianyi Yan1
1College of Biosystems Engineering and Food Science, National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang Engineering Laboratory of Food Technology and Equipment, Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, China.
Abstract:
Chlorella pyrenoidosa (CP) has great potential for feeding future demands in food, environment, energy, and pharmaceuticals. To achieve this goal, the exploitation of emerging efficient technique such as ultrasound-assisted extraction (UAE) for CP nutrient enrichment is crucial. Here, UAE is deployed for high-efficient CP protein (CPP) valorisation. Compared to conventional solvent extraction (CSE), remarkable mass transfer enhancements with 9-time protein yields and 3-time extraction rate are achieved by ultrasonic cavitation in UAE, indicating UAE can drastically shift intracellular nutrients including proteins and pigments to solvent. Cell morphology and ultrastructure show the different responses of cell wall and membrane, indicating that the cell membrane may play a role in the extraction process, based on which the extremely-low efficiency of CSE and high efficiency of UAE are highlighted. This study provides a solution for future food crisis by extracting CPP and may open a new discussion field in ultrasonic extraction.
