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Leaf-venation-directed cellular alignment for macroscale cardiac constructs with tissue-like functionalities.

Mao Mao1,2, Xiaoli Qu1,2, Yabo Zhang1,2

  • 1State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an, 710049, PR China.

Nature Communications
|April 12, 2023
PubMed
Summary
This summary is machine-generated.

This study introduces a leaf-venation-directed method to engineer aligned cardiac tissues. This strategy enhances cell organization, maturation, and functionality for improved cardiac tissue engineering.

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Area of Science:

  • Biomaterials Engineering
  • Tissue Engineering
  • Cardiovascular Research

Background:

  • Replicating native myocardium's complex properties is essential for functional cardiac tissue engineering.
  • Existing methods struggle to achieve the necessary structural, mechanical, and electrophysiological characteristics.

Purpose of the Study:

  • To develop a novel strategy for engineering highly aligned and densely packed cardiac tissues.
  • To improve the maturation and functionality of engineered cardiac tissues using a leaf-venation-directed approach.

Main Methods:

  • A leaf-venation-directed strategy was employed for cell-hydrogel hybrid compaction and remodeling.
  • Hierarchical channels guided cell alignment, forming interconnected tubular structures.
  • Neonatal rat cardiomyocytes and human induced pluripotent stem cell-derived cardiomyocytes were utilized.

Main Results:

  • Leaf-venation-directed tissues exhibited enhanced cell alignment and density compared to random distributions.
  • Engineered tissues showed advanced maturation, detectable electrophysiological activity, and synchronous contractions.
  • 3D centimeter-scale cardiac constructs were assembled with programmed mechanical properties and delivered via tubing without viability loss.

Conclusions:

  • The leaf-venation-directed strategy effectively engineers functional cardiac tissues with improved structural and functional properties.
  • This approach holds promise for generating cardiac constructs with multifaceted functionalities for clinical applications.
  • The method facilitates the creation of centimeter-scale, implantable cardiac tissues.