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Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis
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Berberine Enhances Intestinal Mucosal Barrier Function by Promoting Vitamin D Receptor Activity.

Yong-Quan Huang1,2, Jia-Lin Liu1, Geng-Xin Chen2

  • 1Graduate School, Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.

Chinese Journal of Integrative Medicine
|April 12, 2023
PubMed
Summary

Berberine improves the intestinal barrier in irritable bowel syndrome with diarrhea (IBS-D) by activating vitamin D receptors (VDR). This action enhances tight junction proteins, with the N-terminal region of VDR being key.

Keywords:
Chinese medicineberberineintestinal mucosal barrierirritable bowel syndrome-diarrhea-predominanttight junction proteinsvitamin D receptors

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Area of Science:

  • Gastroenterology
  • Molecular Biology
  • Pharmacology

Background:

  • Irritable bowel syndrome with diarrhea (IBS-D) is characterized by impaired intestinal barrier function.
  • Vitamin D receptor (VDR) plays a role in maintaining intestinal integrity.
  • Berberine is a natural compound with potential therapeutic properties.

Purpose of the Study:

  • To investigate the effect of berberine on VDR activity and tight junction proteins (TJPs) in an IBS-D rat model.
  • To elucidate the role of VDR in berberine-mediated intestinal barrier repair.

Main Methods:

  • An IBS-D rat model was established using neonatal maternal separation and acetic acid stimulation.
  • Rats were treated with varying doses of berberine.
  • Colonic epithelial cells (CECs) were isolated, and VDR activity, VDRE promoter activity, and TJP expression were analyzed.
  • VDR overexpression and silencing techniques were employed.

Main Results:

  • Berberine treatment improved IBS-D symptoms and increased VDRE promoter activity in a dose-dependent manner.
  • Berberine significantly upregulated the expression of TJPs (occludin and zonula occludens-1) in IBS-D CECs.
  • VDR activation, particularly via its N-terminal region, was crucial for berberine's effect on TJPs.

Conclusions:

  • Berberine enhances intestinal mucosal barrier function in IBS-D rats by promoting VDR activity.
  • The N-terminal region of VDR is the primary site mediating berberine's effects on intestinal TJPs.