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Inflammation Control and Tumor Growth Inhibition of Ovarian Cancer by Targeting Adhesion Molecules of E-Selectin

Bowen Yang1,2, Shanmei Yin3, Zishuo Zhou3

  • 1Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu 610041, China.

Cancers
|April 13, 2023
PubMed
Abstract

Insights

E-selectin-binding peptide (ESBP) conjugated nanoparticles target ovarian cancer cells, enhancing drug delivery and inhibiting tumor growth. This targeted approach shows promise for preclinical epithelial ovarian cancer therapy.

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Oncology

Background:

  • Epithelial ovarian cancer (EOC) remains a significant health challenge with limited effective targeted therapies.
  • Targeting adhesion molecules like E-selectin offers a potential strategy for specific cancer cell recognition and drug delivery.
  • Developing novel drug delivery systems (DDS) is crucial for improving therapeutic efficacy and reducing side effects.

Purpose of the Study:

  • To design and evaluate an ovarian cancer-directed drug delivery system utilizing E-selectin-binding peptide (ESBP).
  • To investigate the targeting capabilities and therapeutic effects of ESBP-conjugated bovine serum albumin (BSA) polymerized nanoparticles (ESBP-BSANPs) loaded with paclitaxel (PTX).
  • To elucidate the mechanisms underlying the anti-tumor activity of ESBP-BSANPs-PTX in both in vitro and in vivo models.

Main Methods:

  • Preparation and characterization of BSA polymerized nanoparticles (BSANPs) and ESBP-BSANPs loaded with paclitaxel (PTX).
  • In vitro assessment of nanoparticle targetability (using FITC-labeled nanoparticles) and cytotoxicity (MTT assay) in ovarian cancer cells and relevant cell lines.
  • In vivo evaluation of targeting properties and anti-tumor efficacy in a mouse model of ovarian cancer.

Main Results:

  • ESBP-BSANPs demonstrated enhanced in vitro cellular uptake compared to control nanoparticles.
  • ESBP-BSANPs-PTX exhibited significantly higher cytotoxicity against ovarian cancer cells than PTX alone.
  • In vivo studies confirmed the targeting ability and effective tumor inhibition by ESBP-BSANPs, with increased apoptosis and reduced cell migration.

Conclusions:

  • ESBP-BSANPs-PTX effectively improve paclitaxel targetability, delivering potent, tumor-specific therapeutic activity.
  • The developed drug delivery system shows significant promise for preclinical applications in epithelial ovarian cancer treatment.
  • Targeting E-selectin with ESBP-functionalized nanoparticles represents a viable strategy for enhancing ovarian cancer therapy.
Keywords:
E-selectinbovine serum albuminovarian cancer

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