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Engineered Peptides Enable Biomimetic Route for Collagen Intrafibrillar Mineralization.

Aya K Cloyd1,2, Kyle Boone2,3, Qiang Ye2

  • 1Bioengineering Program, University of Kansas, Lawrence, KS 66045, USA.

International Journal of Molecular Sciences
|April 13, 2023
PubMed
Summary

Dental adhesives struggle with short lifespans. A new peptide approach enables collagen intrafibrillar mineralization, enhancing dental materials for improved durability and clinical performance.

Keywords:
calcium phosphate mineralizationcollagen binding peptidecollagen self-assemblydental cariesdentin adhesive interfacepeptide designresin composites

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Area of Science:

  • Biomaterials Science
  • Dental Materials Science
  • Nanotechnology

Background:

  • Current dental adhesives have limited lifespans due to incomplete collagen fibril enclosure in the hybrid layer.
  • Achieving complete intrafibrillar mineralization of demineralized collagen remains a significant clinical challenge.
  • Developing durable dental restorative materials necessitates innovative approaches to enhance adhesion and longevity.

Purpose of the Study:

  • To develop a peptide-based strategy for achieving collagen intrafibrillar mineralization.
  • To engineer peptides with both collagen-binding and remineralization capabilities.
  • To evaluate the effectiveness of this peptide approach in improving the mechanical properties of collagen fibrils.

Main Methods:

  • Peptide design utilizing domain structure conservation for collagen-binding and remineralization.
  • Structural analysis using attenuated total reflectance Fourier-transform infrared (ATR-FTIR) and circular dichroism (CD) spectroscopy.
  • Assessment of peptide assembly, remineralization, and mechanical properties via atomic force microscopy (AFM) and PeakForce-Quantitative Nanomechanics (PF-QNM)-AFM.

Main Results:

  • Engineered peptides demonstrated successful intrafibrillar mineralization of type-I collagen.
  • Spectroscopic and AFM analyses confirmed peptide assembly and structural changes within collagen fibrils.
  • Remineralized collagen fibrils exhibited enhanced mechanical properties, indicating improved structural integrity.

Conclusions:

  • The peptide-based approach offers a promising method for intrafibrillar collagen remineralization.
  • This strategy can serve as a platform for developing multifunctional dental materials.
  • Further development could lead to improved long-term stability and performance of composite resins.