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Updated: Aug 2, 2025

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Single-Cell Isolation Microfluidic Chip Based on Thermal Bubble Micropump Technology.

Chao Xu1, Kun Wang2, Peng Huang2

  • 1School of Microelectronics, Shanghai University, Shanghai 201800, China.

Sensors (Basel, Switzerland)
|April 13, 2023
PubMed
Summary
This summary is machine-generated.

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This study introduces a novel microfluidic chip for high-throughput single-cell isolation. The chip utilizes thermal bubble micropump technology, enabling efficient isolation for applications like single-cell sequencing and drug development.

Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell Biology

Background:

  • Single-cell isolation is crucial for advanced biological analyses, including single-cell sequencing, monoclonal antibody development, and drug discovery.
  • Traditional methods like flow cytometry (FACS) and laser capture microdissection (LCM) are often complex and low-throughput.
  • There is a need for efficient, high-throughput single-cell isolation techniques to advance cell-based research.

Purpose of the Study:

  • To develop and present a novel microfluidic chip for efficient single-cell isolation.
  • To demonstrate a high-throughput method for isolating individual cells from cell suspensions.
  • To validate the chip's performance using both beads and biological cells.

Main Methods:

  • A microfluidic chip employing thermal bubble micropump technology for fluid manipulation.
Keywords:
microfluidic chipsingle-cell isolationthermal bubble micropump

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Last Updated: Aug 2, 2025

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  • Single-cell isolation achieved by precisely controlling flow resistance within microchannels.
  • Integration of hundreds of single-cell functional modules for parallel processing.
  • Elimination of the need for external injection or peristaltic pumps for cell loading.

    • Main Results:

    • The microfluidic chip demonstrated a near 100% capture rate for single polystyrene beads.
    • The system achieved approximately 75% capture rate for single cells, indicating successful application to biological samples.
    • The integrated design facilitates high-throughput single-cell isolation capabilities.

    Conclusions:

    • The developed microfluidic chip offers a promising, high-throughput solution for single-cell isolation.
    • Thermal bubble micropump technology integrated into microfluidics provides an efficient alternative to traditional methods.
    • This technology has significant potential to accelerate single-cell analysis and related fields.