Isolation of leukocyte-free platelets from standard platelet concentrates by centrifugation

We evaluated centrifugation techniques to isolate leukocyte-free platelets from standard platelet concentrates. Among various settings of centrifugation force and time, we found the setting at 300 X g for 10 minutes or 370 X g for 5 minutes to be efficient and reproducible for recovering sufficient quantities of platelets free of leukocytes.