Concatemeric forms of intracellular Tupaia herpesvirus DNA
Abstract:
Kinetics of Tupaia herpesvirus strain 2 (THV-2) DNA synthesis was measured by labeling with radioactive thymidine and determining the amount of newly synthesized DNA. Viral DNA synthesis reached a maximum between 24 to 36 hr postinfection, preceded by a transient stimulation of host cell DNA synthesis. Blot hybridizations of labeled recombinant plasmids harboring inserts of both genomic termini of THV-2 and of individual viral terminal DNA fragments to DNA of virus-infected cells and combined restriction enzyme analysis of labeled intracellular viral DNA revealed the presence of linear concatemeric and/or circular viral DNA molecules.
More Related Videos
08:26Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins
Published on: August 31, 2017
09:15Tyramide Signal Amplification for the Immunofluorescent Staining of ZBP1-Dependent Phosphorylation of RIPK3 and MLKL After HSV-1 Infection in Human Cells
Published on: October 20, 2022
Related Concept Videos
Retrovirus Life Cycles
Viral Recombination
Retroviruses
LTR Retrotransposons
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
DNA Bacteriophages
Herpes
