[Determination of free amino acids in body tissues]
Related Experiment Video
Updated: Sep 23, 2026

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
Published on: January 26, 2012
Abstract:
The authors describe methods for isolation and assay of free amino acids in body tissues employed today in clinical and experimental studies. Give an account of the main factors affecting the data obtained. Compare the efficacy of different methods for quantitative determination of free amino acids in tissues. Analyse difficulties arising in the option of the deproteinizing agent while preparing tissue samples to be tested. Based on the reported data and their own experience the authors suggest efficacious and less time-consuming research methods for assay of free amino acids in blood plasma, red blood cells, and tissue homogenates.

