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Related Experiment Video

Updated: Aug 2, 2025

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures
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Millimeter-thick 3D tissues constructed by densely cellularized core-shell microfluidic bioprinting.

Minghao Nie1, Shogo Nagata2,3, Haruka Oda1

  • 1Department of Mechano-Informatics, Graduate School of Information Science and Technology, The University of Tokyo, Tokyo, Japan.

Biofabrication
|April 14, 2023
PubMed
Summary

This study introduces a novel microfluidic bioprinting technique to create densely cellularized 3D tissue constructs. The method enables the fabrication of tissue with cell densities comparable to native solid organs.

Keywords:
HepG2cell-laden microfibercore–shell microfiberdynamic tissue culturemicrofluidic bioprinting

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Last Updated: Aug 2, 2025

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Area of Science:

  • Biotechnology
  • Tissue Engineering
  • Microfluidics

Background:

  • Microfluidic bioprinting offers high resolution but struggles to create densely cellularized tissues.
  • Achieving firm tissue consistency requires high cell density, crucial for solid-organ biofabrication.

Purpose of the Study:

  • To develop a microfluidic bioprinting method for fabricating densely cellularized 3D tissue constructs.
  • To encapsulate cells and extracellular matrices within core-shell microfibers for improved tissue formation.

Main Methods:

  • Utilized a novel microfluidic bioprinting approach with optimized printhead design and parameters.
  • Fabricated core-shell microfibers encapsulating cells and extracellular matrices.
  • Cultured 3D constructs using dynamic culture methods and assessed cell viability, morphology, and function.

Main Results:

  • Successfully bioprinted macroscale constructs using core-shell microfibers with high cell viability.
  • Demonstrated confluent tissue morphology and intensive cell-cell contacts within fiber cores.
  • Achieved cell densities comparable to in vivo solid organ tissues, with upregulated albumin secretion.

Conclusions:

  • The developed microfluidic bioprinting method effectively creates densely cellularized 3D tissue constructs.
  • The technique supports robust cell viability, tissue formation, and functional maturation.
  • Future improvements in culture techniques may enable thicker tissue fabrication for therapeutic applications.