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Updated: Aug 2, 2025

Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice
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Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice

Published on: June 8, 2022

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Transcriptome landscape reveals the chronic inflammatory response in kidneys affected by the combinatory effect of

Li-Fang Chou1, Chih-Wei Huang2, Huang-Yu Yang3

  • 1Kidney Research Center and Department of Nephrology, Chang Gung Memorial Hospital, Linkou, Taiwan; Graduate Institute of Biomedical Sciences, Department of Microbiology and Immunology, Department of Biochemistry, Chang Gung University, Taoyuan, Taiwan.

Genomics
|April 16, 2023
PubMed

Insights

Chronic leptospirosis combined with secondary kidney injury accelerates chronic kidney disease (CKD) progression. This study reveals key gene disruptions and immune pathways involved in this accelerated renal damage.

Area of Science:

  • Nephrology
  • Immunology
  • Microbiology

Background:

  • Leptospirosis can lead to chronic kidney damage.
  • Secondary kidney injuries exacerbate renal failure risk in patients.
  • Understanding combined insults is crucial for managing progressive chronic kidney disease (CKD).

Purpose of the Study:

  • To investigate the combined effects of leptospirosis and secondary kidney injury on kidney transcriptome.
  • To identify molecular mechanisms underlying accelerated CKD progression.
  • To discover potential diagnostic or therapeutic targets for combined renal injury.

Main Methods:

  • Transcriptome profiling of mouse kidneys.
  • Analysis of adenine-induced and chronic Leptospira-infected kidney models.
  • Assessment of gene expression and protein levels, including Indoleamine 2,3-dioxygenase 1.

Main Results:

  • Combined injury activated chronic inflammation and T-helper 17 immune responses.
  • High expression of Indoleamine 2,3-dioxygenase 1 protein was observed.
  • Significant gene expression disruptions were identified, indicating predisposition to CKD symptoms.

Conclusions:

  • The combination of leptospirosis and secondary nephrotoxic injury promotes chronic inflammation and kidney dysfunction.
  • Immunometabolic dysregulation plays a role in the observed renal injury.
  • Disrupted genes identified may serve as novel targets for CKD diagnosis and treatment.
Keywords:
AdenineChronic kidney diseaseCombinatory effectLeptospirosisRenal transcriptome

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