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Altered plasma exosome miRNAs and novel potential biomarkers in pediatric fulminant myocarditis

Xinyue Zhang1, Ruling Yang1, Mengjie Ma1

  • 1Department of Pediatric Cardiology, Shandong Provincial Hospital, Shandong University, Jinan, China.

Genomics
|April 16, 2023
PubMed

Insights

Exosomes carrying microRNAs (miRNAs) impact fulminant myocarditis (FM) development. Plasma exosome miRNA profiles in pediatric FM revealed specific biomarkers, including hsa-miR-146a-5p, offering diagnostic potential.

Area of Science:

  • Cardiovascular Research
  • Immunology
  • Molecular Biology

Background:

  • Exosomes mediate intercellular communication via microRNAs (miRNAs), influencing diseases like fulminant myocarditis (FM).
  • Understanding miRNA profiles in pediatric FM is crucial for elucidating disease mechanisms.

Purpose of the Study:

  • To investigate the plasma exosome miRNA profile in pediatric FM.
  • To identify specific miRNA biomarkers and regulatory networks involved in FM pathogenesis.

Main Methods:

  • Small RNA microarray analysis of plasma exosomes from pediatric FM patients.
  • Bioinformatic analysis to identify differentially expressed miRNAs and target genes.
  • Quantitative real-time PCR (qRT-PCR) validation of specific miRNAs.

Main Results:

  • Identified 266 differentially expressed miRNAs (197 upregulated, 69 downregulated) in pediatric FM exosomes.
  • Established competing endogenous RNA (ceRNA) networks involving lncRNAs, circRNAs, miRNAs, and mRNAs.
  • Validated elevated expression of hsa-miR-146a-5p, hsa-miR-23a-3p, and hsa-miR-27a-3p; hsa-miR-146a-5p demonstrated high diagnostic sensitivity and specificity.

Conclusions:

  • Plasma exosome miRNAs play a significant regulatory role between immune and cardiac cells in pediatric FM.
  • Specific exosome miRNAs, particularly hsa-miR-146a-5p, serve as potential diagnostic biomarkers for FM.
  • This study provides novel research targets for understanding and treating pediatric FM.
Keywords:
BiomarkerExosomeFulminant myocarditisImmuneInflammationmicroRNA

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