Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A physics-informed alternative to Richardson-Lucy deconvolution across SNR regimes without iteration cutoffs.

Nature communications·2026

Same author

Mitochondria directly interact with the nuclear pore complex.

Nature·2026

Same author

Stochastic colonization and host-to-host transmission shape gut bacterial variability.

bioRxiv : the preprint server for biology·2026

Same author

Resolving fluorescently labeled species using highly multiplexed spectral FLIM.

Scientific reports·2026

Same author

Simulation-based inference captures non-Markovian effects as exemplified in protein production kinetics through cell division.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Substrate-interacting pore loops of two ATPase subunits determine the degradation efficiency of the 26S proteasome.

Nature communications·2026

Same journal

Layered social competition coordinates reproductive hierarchy formation in ants.

bioRxiv : the preprint server for biology·2026

Same journal

Combination epigenetic-targeted therapy increases the immunogenicity of poorly immunogenic sarcomas.

bioRxiv : the preprint server for biology·2026

Same journal

Loss of LanC-like proteins delays post-injury regeneration of aging skeletal muscles.

bioRxiv : the preprint server for biology·2026

Same journal

Integrative Transfer Network: Deep Transfer Learning Across Populations and Prediction Targets.

bioRxiv : the preprint server for biology·2026

Same journal

Confidence-supported label-free metabolic imaging with FPhaS phase autofluorescence microscopy.

bioRxiv : the preprint server for biology·2026

Same journal

Sequence-encoded autoinhibition couples mRNA decapping activity to phase separation.

bioRxiv : the preprint server for biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Aug 2, 2025

A Protocol for Real-time 3D Single Particle Tracking

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

BNP-Track: A framework for multi-particle superresolved tracking.

Lance W Q Xu^1,2, Ioannis Sgouralis³, Zeliha Kilic⁴

¹Center for Biological Physics, Department of Physics, Arizona State University, Tempe, AZ 85287, USA.

Biorxiv : the Preprint Server for Biology

|April 17, 2023

Summary

This summary is machine-generated.

We developed BNP-Track, a novel Bayesian nonparametric tracking tool that accurately tracks multiple fluorescent molecules by analyzing spatiotemporal correlations, outperforming conventional methods for dynamic targets.

More Related Videos

Image-based Lagrangian Particle Tracking in Bed-load Experiments

Image-based Lagrangian Particle Tracking in Bed-load Experiments

Published on: July 20, 2017

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

Published on: July 25, 2014

Related Experiment Videos

Last Updated: Aug 2, 2025

A Protocol for Real-time 3D Single Particle Tracking

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

Image-based Lagrangian Particle Tracking in Bed-load Experiments

Image-based Lagrangian Particle Tracking in Bed-load Experiments

Published on: July 20, 2017

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

Published on: July 25, 2014

Area of Science:

Microscopy and biophysics
Computational biology and data analysis
Superresolution imaging

Background:

Tracking fluorescent molecules is challenging due to point spread function overlap, especially in crowded environments.
Existing superresolution methods struggle with dynamic targets due to temporal delays.
Neighboring molecule information is encoded in spatiotemporal intensity correlations.

Approach:

Developed BNP-Track, a Bayesian nonparametric tool for simultaneous inference of emitter number and tracks.
Utilized spatiotemporal correlations for superresolved tracking of dynamic targets.
Tested BNP-Track's robustness across parameter regimes and compared it to competing methods.

Key Points:

BNP-Track achieves superresolved tracking by leveraging all encoded spatiotemporal correlations.
Stochastic background treatment enhances emitter number determination accuracy.
BNP-Track corrects for point spread function blur and propagates various error sources.

Conclusions:

BNP-Track extends the superresolution paradigm to dynamic targets.
The tool can track multiple diffraction-limited emitters unresolved by conventional methods.
BNP-Track offers superior accuracy and robustness in complex molecular tracking scenarios.