Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Thermal stability of P-loaded Li-LSX zeolites for air separation.

RSC advances·2026

Same author

Prognostic utility of circulating tumor DNA assessment in immune checkpoint inhibitor-treated advanced non-small cell lung cancer: a systematic review and meta-analysis.

Frontiers in immunology·2026

Same author

Guilu Erxian Glue Restores Immune Homeostasis in Aplastic Anemia Mice by Regulating Treg Lineage Stability via the miRNA-17/10a and FasL/Fas Signaling Pathways.

Journal of inflammation research·2026

Same author

Prevalence of Getah Virus in Mammals in East and Southeast Asia: A Systematic Review and Meta-Analysis.

Transboundary and emerging diseases·2026

Same author

Decoupling of global metabolic flux and proteome partitioning in bacteria.

Science (New York, N.Y.)·2026

Same author

Comprehensive assessment of on- and off-target mutagenesis via lipid nanoparticle delivery of CRISPR-Cas9 genome editing.

Molecular therapy. Nucleic acids·2026

Same journal

A human-specific genetic modifier reconfigures large-scale cortical network dynamics underlying behavioral performance.

bioRxiv : the preprint server for biology·2026

Same journal

<i>Staphylococcus aureus</i> uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis.

bioRxiv : the preprint server for biology·2026

Same journal

Dynamic redistribution of eIF4F controls cap-dependent translation initiation.

bioRxiv : the preprint server for biology·2026

Same journal

When does additional information improve accuracy of RNA secondary structure prediction?

bioRxiv : the preprint server for biology·2026

Same journal

Normative brain-state trajectories reveal deviation from healthy aging in Alzheimer's disease.

bioRxiv : the preprint server for biology·2026

Same journal

Noradrenergic infraslow rhythm during sleep is the critical link between heart-rate dynamics and memory consolidation.

bioRxiv : the preprint server for biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Aug 2, 2025

High-Throughput Live Imaging of Microcolonies to Measure Heterogeneity in Growth and Gene Expression

High-Throughput Live Imaging of Microcolonies to Measure Heterogeneity in Growth and Gene Expression

Published on: April 18, 2021

Tools and methods for high-throughput single-cell imaging with the mother machine.

Ryan Thiermann¹, Michael Sandler¹, Gursharan Ahir¹

¹Department of Physics, University of California San Diego, La Jolla CA.

Biorxiv : the Preprint Server for Biology

|April 17, 2023

Summary

This summary is machine-generated.

We developed napari-MM3, an accessible image analysis tool for mother machine microscopy. Key single-cell measurements are robust across pipelines, but parameter choices can introduce bias, especially with deep learning segmentation.

More Related Videos

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

Published on: April 30, 2019

Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques

Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques

Published on: June 30, 2017

Related Experiment Videos

Last Updated: Aug 2, 2025

High-Throughput Live Imaging of Microcolonies to Measure Heterogeneity in Growth and Gene Expression

High-Throughput Live Imaging of Microcolonies to Measure Heterogeneity in Growth and Gene Expression

Published on: April 18, 2021

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

Published on: April 30, 2019

Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques

Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques

Published on: June 30, 2017

Area of Science:

Microscopy and image analysis
Computational biology
Cell biology

Background:

High-throughput analysis of microscopy data, particularly single-cell time-lapse imaging using the mother machine platform, faces significant bottlenecks in image processing.
Existing mother machine image analysis pipelines often lack user-friendliness for non-expert researchers, hindering widespread adoption.
Quantitative comparisons of different mother machine data analysis pipelines are scarce, limiting understanding of method-specific biases.

Conclusions:

napari-MM3 provides a user-friendly and effective solution for mother machine image analysis, leveraging napari's interactive capabilities.
While core physiological measurements are robust, careful parameter selection and validation are crucial to avoid systematic biases in single-cell analysis.
The study highlights the importance of understanding data-model interactions in deep learning for accurate microbial cell imaging analysis.