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pH microelectrode: modified Thomas recessed-tip configuration.

L R Pucacco, S K Corona, H R Jacobson

    Analytical Biochemistry
    |March 1, 1986
    PubMed
    Summary
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    A new modified Thomas pH microelectrode was developed for measuring intracellular pH in small cells. This innovative electrode offers low resistance, a tiny sensing chamber, and fast response times for precise biological measurements.

    Area of Science:

    • Biomedical Engineering
    • Cell Biology
    • Electrochemistry

    Background:

    • Accurate measurement of intracellular pH is crucial for understanding cellular function.
    • Existing microelectrodes may have limitations in sensitivity or applicability to small cells.
    • Mammalian kidney tubules present a challenge for intracellular pH monitoring due to cell size.

    Purpose of the Study:

    • To develop a novel pH microelectrode suitable for measuring intracellular pH in small mammalian cells.
    • To overcome limitations of existing technologies for micro-scale pH measurements.
    • To facilitate research in kidney tubule physiology and cellular energetics.

    Main Methods:

    • Development of a modified Thomas pH microelectrode using a glass-membrane pH electrode.

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  • Incorporation of a water-tight seal for enhanced stability and performance.
  • Characterization of electrode properties including electrical resistance, sensing chamber volume, and response time.
  • Manufacturing of both single- and double-barreled configurations.

    • Main Results:

    • The modified Thomas electrode exhibits low electrical resistance (10^11 omega).
    • It features a small sensing chamber (10 microns^3) for precise measurements.
    • The electrode demonstrates a rapid response time of 10 seconds.
    • The design allows for both single- and double-barreled configurations.

    Conclusions:

    • The modified Thomas pH microelectrode is a viable tool for intracellular pH measurement in small cells.
    • Its characteristics make it particularly suitable for applications in mammalian kidney tubules.
    • This development advances the capability for studying cellular physiology at the micro-scale.