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Related Experiment Videos

Lowry protein assay using an automatic microtiter plate spectrophotometer.

H J Fryer, G E Davis, M Manthorpe

    Analytical Biochemistry
    |March 1, 1986
    PubMed
    Summary

    This study adapts the Lowry protein assay for 96-well microplates, enabling faster, more efficient protein quantification using smaller sample volumes. The automated system simplifies analysis for diverse protein concentrations.

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    Area of Science:

    • Biochemistry
    • Analytical Chemistry
    • Laboratory Science

    Background:

    • The Lowry protein assay is a widely used method for protein determination.
    • Traditional Lowry assays can be time-consuming and require larger sample volumes.
    • Adaptation to microplate formats is needed for high-throughput analysis.

    Purpose of the Study:

    • To adapt the Lowry protein determination method for 96-well microtiter plates.
    • To integrate an automatic microplate spectrophotometer with computer analysis for protein quantification.
    • To adapt the Bensadoun and Weinstein protein precipitation method for microplate use.

    Main Methods:

    • Adaptation of the Lowry protein assay for 96-well microplates.
    • Utilizing an automatic microplate spectrophotometer interfaced with a computer.

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  • Incorporating a modified Bensadoun and Weinstein method for protein precipitation.

    • Main Results:

    • The adapted method is faster and requires smaller sample volumes (100 microliters).
    • It accurately quantifies protein concentrations ranging from 3-300 micrograms/ml (0.3-30 micrograms/assay).
    • The system automatically plots standard curves and calculates sample protein content.

    Conclusions:

    • The adapted microplate Lowry assay offers significant advantages in speed and sample volume reduction.
    • This automated technique is highly suitable for laboratories processing numerous samples with varying protein concentrations.
    • The integration of precipitation and assay methods enhances its utility for complex samples.