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High ciliary neuronotrophic specific activity in rat peripheral nerve.

L R Williams¹, M Manthorpe¹, G Barbin¹

¹Department of Biology, School of Medicine, University of California, San Diego, La Jolla, CA 92093, U.S.A.

International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience

|May 31, 2014

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Ciliary neuronotrophic factors (CNTF) promote neuron survival. Extracts from adult rat peripheral nerves and spinal nerve roots show high CNTF activity, comparable to intraocular tissues.

Area of Science:

Neuroscience
Cell Biology
Biochemistry

Background:

Ciliary neuronotrophic factors (CNTF) are proteins crucial for neuron survival.
CNTF has been identified in various biological sources.
Intraocular tissue historically shows the highest specific CNTF activity.

Purpose of the Study:

To investigate CNTF presence and activity in mammalian peripheral nervous system.
To compare CNTF levels in peripheral nerves with known sources like intraocular tissue.

Main Methods:

Extraction of proteins from adult rat peripheral nerve and spinal nerve roots.
Assay of Ciliary Neuronotrophic Factor (CNTF) activity in tissue extracts.
Quantification of specific CNTF activity (TU/mg).

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Main Results:

Adult rat peripheral nerve and spinal nerve roots contain significant CNTF activity.
Specific CNTF activity in these nerve tissues is equal to or greater than intraocular tissue.
High CNTF concentrations were found in both motor and sensory nerve types.

Conclusions:

Mammalian peripheral nerves are a rich source of Ciliary Neuronotrophic Factor (CNTF).
This finding expands the known distribution of CNTF in the nervous system.
The cellular origins of mammalian CNTF in peripheral nerves warrant further investigation.