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Related Experiment Videos

A sequential, solid-phase assay for biotin in physiologic fluids that correlates with expected biotin status.

D M Mock, D B DuBois

    Analytical Biochemistry
    |March 1, 1986
    PubMed
    Summary

    This study introduces a new, highly sensitive biotin assay using radiolabeled avidin. The method accurately measures biotin levels in plasma and urine, aiding in diagnosing metabolic disorders and deficiencies.

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    Area of Science:

    • Biochemistry
    • Clinical Chemistry
    • Metabolic Disorders

    Background:

    • Growing interest in accurate biotin measurement due to biotin-responsive inborn errors of metabolism.
    • Concerns regarding acquired biotin deficiency from parenteral alimentation.

    Purpose of the Study:

    • To develop and validate a sensitive biotin assay for plasma and urine.
    • To improve diagnostic capabilities for biotin-related metabolic conditions.

    Main Methods:

    • A sequential, solid-phase biotin assay.
    • Utilized radiolabeled avidin for detection.
    • Validated using a rat model of biotin deficiency.

    Main Results:

    • Achieved high sensitivity (≥ 10 fmol/tube).

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  • Demonstrated correlation with expected biotin concentration trends in a deficiency model.
  • Confirmed utility with commercially available radiolabeled avidin.

    • Conclusions:

    • The developed assay offers enhanced sensitivity and accuracy for biotin measurement.
    • This method supports the diagnosis and management of biotin metabolism disorders.
    • Provides a reliable tool for clinical and research applications.