Bactericidal activity of Ag nanoparticles biosynthesized from Capsicum annuum pericarps against phytopathogenic...

Metallic nanoparticles of different compositions have already found numerous applications in various branches of industry, agriculture, and medicine. Given the well-known antibacterial activity of Ag, silver nanoparticles (AgNPs) are constantly being investigated for their promising ability to fight antibiotic-resistant pathogens. A promising candidate for AgNPs biosynthesis is chili pepper Capsicum annuum, cultivated worldwide and known for accumulating significant amounts of active substances. Phytochemical screening of aqueous extract of C. annuum pericarps demonstrated accumulation of 4.38 mg/g DW of total capsaicinoids, 14.56 mg GAE/g DW of total phenolic compounds, 1.67 mg QE/g DW of total flavonoids, and 1.03 mg CAE/g DW of total phenolic acids. All determined aromatic compounds carry various active functional groups, which effectively participate in the biosynthesis of AgNPs and are characterized by high antioxidant potential. Therefore, the present research focused on the facile, quick, and effective procedure for the biosynthesis of AgNPs, which were analyzed for their morphology such as shape and size through UV-visible, Fourier-transform infrared spectroscopy (FTIR) assays, and scanning electron microscopy. We found that the AgNPs biosynthesis resulted in changes in FTIR spectra, depicting the rearrangement of numerous functional groups, while the nanoparticles themselves were shown to be stable, spherical, 10-17 nm in size. Also we investigated the antibacterial properties of biosynthesized AgNPs, obtained with C. annuum fruit extracts, against a common phytopathogen Clavibacter michiganensis subsp. michiganensis. As was shown by zone inhibition assay, AgNPs showed dose-dependent 5.13-6.44 cm antibacterial activity, greatly exceeding the 4.98 cm inhibition area, produced by the precursor salt, AgNO₃.