Oral Nanoarmored Lactobacillus rhamnosus with Inflammation Targeting and Scavenging Activities for Colitis...

Nanoarmored probiotics with inflammation targeting and scavenging activities without complex synthetic procedure is important for maintaining their native bioactivity, yet it remains a challenge. In this work, we demonstrated the multifunctional nanoparticle (QL@PS NP)-armored Lactobacillus rhamnosus (LAB) with inflammation targeting and regulation activity for inflammatory bowel disease (IBD) treatment. QL@PS NPs were assembled by quercetin (Que), lipoic acid (LA), and phosphatidylserine (PS), which could target macrophages and degrade in reactive oxygen species (ROS)-rich lesion sites, exhibiting excellent antioxidant and anti-inflammatory capability in vitro. QL@PS NPs were coated on the surface of thiolated LAB under a cell-friendly condition, and the resulting nanoarmored probiotic (LAB@NPs) could protect LAB from the gastrointestinal assaults and ROS damage. LAB@NPs are more likely to be up-taken by macrophages, scavenging oxidative stress and regulating the inflammatory microenvironment of colitis lesions through the synergistic effect of probiotics and QL@PS NPs. In vivo experimental results certified that LAB@NPs achieved notable lesion-specific therapeutic efficacy in mouse colitis models, significantly ameliorating colon shortening, reducing inflammatory reactions and pathological tissue damage. This work provides a facile and effective strategy to construct nanoarmored probiotics for therapy of IBD.