Related Experiment Video
Updated: Aug 2, 2025
Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug Discovery
Published on: April 26, 2024
A co-culture model system to quantify antibody-dependent cellular cytotoxicity in human breast cancer cells using an
Roos Vincken1, Ana Ruiz-Saenz2
1Department of Cell Biology, Erasmus University Medical Center Rotterdam, 3015 CN Rotterdam, the Netherlands.
Abstract:
Current protocols measure antibody-dependent cellular cytotoxicity (ADCC) in vitro using peripheral blood mononuclear cells (PBMCs), but isolation and variability among donors limit the viability and reproducibility of this approach. Here, we present a standardized co-culture model system to quantify ADCC on human breast cancer cells. We describe steps to engineer a natural killer cell line that stably expresses FCγRIIIa (CD16), required to mediate ADCC. We then detail the steps for the cancer-immune co-culture setup, followed by cytotoxicity measurement and analysis.
