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Dorfman pooling enhances SARS-CoV-2 large-scale community testing efficiency.

Julian Burtniak1, Adam Hedley1,2, Kerry Dust2

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Pooled testing for SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) using Dorfman strategies enhances testing capacity. This method improves resource efficiency in diagnostic laboratories, especially in low-resource settings.

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Area of Science:

  • Clinical diagnostics
  • Molecular biology
  • Epidemiology

Background:

  • Polymerase chain reaction (PCR) is the standard for SARS-CoV-2 detection.
  • High demand during the pandemic strained diagnostic resources and testing capacity.
  • Pooled testing offers a solution to increase testing capacity and conserve resources.

Purpose of the Study:

  • Analyze SARS-CoV-2 pooling schemes, specifically Dorfman strategies.
  • Determine the sensitivity of various Dorfman pool sizes.
  • Evaluate the utility of pooling for diagnostic laboratories.

Main Methods:

  • Assessed sensitivity across different Dorfman pool sizes.
  • Calculated efficiency based on test positivity rates.
  • Correlated findings with current positivity rates to optimize resource savings.

Main Results:

  • A trend of decreasing sensitivity with increasing pool size was observed.
  • Modest sensitivity losses occurred in the largest pools, with high sensitivity in smaller pools.
  • Optimal Dorfman pool sizes were identified to maximize test savings and efficiency.

Conclusions:

  • Dorfman pooling is a high-throughput solution for SARS-CoV-2 clinical testing.
  • Pooling strategies significantly improve resource efficiency, particularly in low-resource environments.
  • This approach enhances overall testing capacity and community-level resource management.