Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Mitotic Cdc42 waves encode PI(3,4)P<sub>2</sub> signaling and Golgi morphological state to control spindle scaling.

Science advances·2026

Same author

Label-Free and High-Throughput Quantification of Nanoparticle-Cell Interactions at the Single-Cell Level with Flow Cytometry.

Analytical chemistry·2026

Same author

Promoting Self-Efficacy of Biomedical Engineering Undergraduate Students Using a Deliberately Designed Nanomedicine Workshop Series.

Biomedical engineering education·2026

Same author

YES Oklahoma: Building Pathways into Cancer Research and Public Health for Indigenous High School Students.

Journal of cancer education : the official journal of the American Association for Cancer Education·2026

Same author

Lipid Nanoparticle Surface Engineering with Heparosan Polysaccharides for Safe and Effective mRNA Delivery <i>In Vitro</i> and <i>In Vivo</i>.

ACS applied materials & interfaces·2026

Same author

Implementation of an adaptive-optics assisted isoSTED nanoscope.

Nature protocols·2026

Same journal

A pH-Tolerant Nickel-Vanadium Phosphonate Framework for Stable Aqueous Supercapacitor Cycling.

ACS nano·2026

Same journal

Reconfigurable Photoelectric Coaxial Fiber-Based Memristors for Neuromorphic Computing.

ACS nano·2026

Same journal

Multidimensional Emission Control of CsPbI<sub>3</sub> Quantum Dots Using Plasmonic Quasi-Bound States in the Continuum.

ACS nano·2026

Same journal

Reconfigurable 2D Floating-Gate Field-Effect Transistors with Graphene-Induced Interfacial Polarization for Unified Memory-Logic Integration.

ACS nano·2026

Same journal

Bioinstructive Hybrid Scaffold Integrating Phosphoinositide 3-Kinase-Akt and Complementary Survival Pathways for Kidney Regeneration.

ACS nano·2026

Same journal

Robust Quantum Cutting via Halide-Bearing Ligand Passivation and Gradient Halide Reconstruction for Ultrabroadband Ultraviolet-to-Near-Infrared Photodetection and Imaging.

ACS nano·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Aug 2, 2025

Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions

Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions

Published on: August 26, 2021

Quantifying Intracellular Nanoparticle Distributions with Three-Dimensional Super-Resolution Microscopy.

Vinit Sheth¹, Xuxin Chen², Evan M Mettenbrink¹

¹Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, Oklahoma 73019, United States.

|April 18, 2023

Summary

This summary is machine-generated.

This study introduces a novel super-resolution imaging technique for visualizing nanoparticle distribution within cells. This method enables detailed 3D imaging of intracellular nanoparticles, aiding nanomedicine development.

Keywords:

3D imaging expansion microscopy label-free nanomedicine nanoparticles super-resolution

More Related Videos

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes

Published on: May 24, 2019

Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry

Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry

Published on: December 12, 2013

Related Experiment Videos

Last Updated: Aug 2, 2025

Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions

Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions

Published on: August 26, 2021

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes

Published on: May 24, 2019

Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry

Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry

Published on: December 12, 2013

Area of Science:

Nanotechnology
Cell Biology
Microscopy

Background:

Understanding nanoparticle-cell interactions is crucial for nanomedicine.
Current imaging techniques have limitations in visualizing intracellular nanoparticle distribution.

Purpose of the Study:

To develop a super-resolution imaging technology for visualizing nanoparticle distribution inside mammalian cells.
To enable quantitative 3D imaging of intracellular nanoparticles with high resolution.

Main Methods:

Cells exposed to metallic nanoparticles were embedded in swellable hydrogels.
Quantitative label-free imaging utilizing nanoparticle light scattering properties.
Expansion microscopy protocols (protein retention and pan-expansion) were validated.

Main Results:

Achieved quantitative 3D imaging of intracellular nanoparticles with electron-microscopy-like resolution.
Demonstrated label-free imaging with ultrastructural context.
Validated nanoparticle uptake studies and determined 3D spatial distribution within single cells.

Conclusions:

The developed super-resolution imaging platform allows detailed visualization of intracellular nanoparticle fate.
This technology can inform the engineering of safer and more effective nanomedicines.
Broad applications in fundamental and applied nanomedicine research.