Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mechanical Properties, Hydration Mechanisms, and Microwave-Absorbing Properties of Alkali-Activated Blast-Furnace Slag Containing Steel Slag.

Materials (Basel, Switzerland)·2026
Same author

Nomograms Predictive of Recurrence in Malignant Tumors Treated With Skull Base Surgery: An International Collaborative Study.

Head & neck·2026
Same author

The impact of virtual reality acute aerobic exercise on the inhibitory control function of anxious college students: An exploratory neurological mechanism study based on fNIRS.

Psychology of sport and exercise·2026
Same author

Experimental verification and PK/PD modeling of selective drug absorption via acupoint administration in rabbit model of rheumatoid arthritis.

Frontiers in neurology·2026
Same author

Digestive behavior of lactoferrin-pectin electrostatic complexes: effect of pectin esterification degree on the enhancement of gastric stability.

Journal of dairy science·2026
Same author

Electrosurgical Leaflet Modification in TAVR: Adoption and Adaptation Across the Asia-Pacific Region.

JACC. Asia·2026

Related Experiment Video

Updated: Jun 11, 2026

Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry
07:31

Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry

Published on: March 28, 2014

Label-Free and High-Throughput Quantification of Nanoparticle-Cell Interactions at the Single-Cell Level with Flow

Mobina Mohammadnejad1, Majood Haddad1,2, Alex N Frickenstein1

  • 1Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, Oklahoma 73019, United States.

Analytical Chemistry
|June 10, 2026
PubMed
Summary

Flow cytometry enables label-free quantification of nanoparticle-cell interactions at the single-cell level. This method aids in designing safer and more effective nanomedicines by analyzing nanoparticle uptake kinetics.

More Related Videos

Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry
14:09

Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry

Published on: December 12, 2013

Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry
08:26

Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry

Published on: April 6, 2022

Related Experiment Videos

Last Updated: Jun 11, 2026

Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry
07:31

Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry

Published on: March 28, 2014

Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry
14:09

Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry

Published on: December 12, 2013

Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry
08:26

Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry

Published on: April 6, 2022

Area of Science:

  • Nanomedicine
  • Biotechnology
  • Cellular Biology

Background:

  • Understanding nanoparticle-cell interactions is crucial for developing advanced nanomedicines.
  • Current methods for quantifying these interactions can be limited in throughput and scope.

Purpose of the Study:

  • To establish flow cytometry as a label-free technique for single-cell quantification of nanoparticle-cell interactions.
  • To analyze how nanoparticle properties influence cellular uptake and interactions.
  • To enable high-throughput analysis of nanoparticle-cell dynamics.

Main Methods:

  • Utilized conventional flow cytometry side-scattering signals for label-free quantification.
  • Corroborated findings with optical super-resolution microscopy and elemental mass spectrometry.
  • Developed a multiparameter workflow analyzing over 10,000 single cells per minute.

Main Results:

  • Successfully quantified nanoparticle-cell interactions and uptake kinetics at the single-cell level.
  • Demonstrated the workflow's applicability to various nanoparticle characteristics (size, composition, surface chemistry, concentration).
  • Validated findings using super-resolution expansion microscopy and single-particle inductively coupled plasma mass spectrometry.

Conclusions:

  • Flow cytometry provides a powerful, high-throughput method for quantitatively assessing nanoparticle-cell interactions.
  • This approach facilitates the rational design of next-generation nanomedicines with improved safety and efficacy.
  • The workflow is adaptable to complex in vitro cell models, including mixed-cell and coculture systems.