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Citrate phosphate dextrose adenine (CPDA-1) can be sampled from blood collection bags in a multi-dose manner
Cheryl M Stout1, Jessie M Campanile2, S Emmanuelle Knafo1
11Department of Avian and Exotics, Red Bank Veterinary Hospital, Tinton Falls, NJ.
Insights
Citrate phosphate dextrose adenine (CPDA-1) blood collection bags remain sterile for up to 20 days when sampled aseptically, supporting multi-dose use and reducing waste.
Area of Science:
- Transfusion Medicine
- Microbiology
- Blood Banking
Background:
- Multi-dose sampling from blood collection bags is common practice.
- Maintaining the sterility of anticoagulant solutions is crucial for patient safety.
- Citrate phosphate dextrose adenine (CPDA-1) is a widely used anticoagulant.
Purpose of the Study:
- To assess the sterility of CPDA-1 anticoagulant in blood collection bags when subjected to multi-dose sampling.
- To determine the impact of storage temperature on the sterility of CPDA-1 over time.
Main Methods:
- 10 CPDA-1 blood collection bags were stored at 24°C or 5°C for 30 days.
- Aseptic 1.0 mL aliquots were withdrawn every 5 days for bacterial culture and every 10 days for fungal culture.
- Cultures were analyzed for microbial growth.
Main Results:
- Two positive microbial isolates were identified: Bacillus on day 0 and Candida on day 30.
- These isolates are suspected to be due to post-sampling contamination.
- All other samples remained negative for microbial growth.
Conclusions:
- CPDA-1 blood collection bags can be safely used in a multi-dose manner for up to 20 days if samples are obtained aseptically.
- This supports the clinical practice of multiple withdrawals from a single bag, minimizing discard.
Objective:
To evaluate the sterility of citrate phosphate dextrose adenine (CPDA-1) anticoagulant when sampled from blood collection bags in a multi-dose manner.
Sample:
10 pre-filled CPDA-1 blood collection bags; 46 bacterial and 28 fungal culture result reports.
Procedures:
10 CPDA-1 blood collection bags were split into 2 equal groups and stored at either room temperature (24 °C) or refrigerator temperature (5 °C) for 30 days. Two bags in each group were designated as controls. Beginning on day 0 a 1.0 mL aliquot was withdrawn from each experimental bag and submitted for bacterial culture (aerobic and anaerobic) every 5 days, and fungal culture every 10 days. All 10 bags were sampled on day 30. Bacterial and fungal culture results were compiled and interpreted.
Results:
46 CPDA-1 aliquots were cultured, resulting in 2 positive microbial isolates: Bacillus was cultured from a previously unopened experimental bag on day 0, and Candida was cultured from a refrigerated experimental bag on day 30. Both positives are thought to represent post-sampling contamination, though these suspicions cannot be confirmed in the bag yielding Candida due to a lack of subsequent data. All other samples were negative for microbial growth.
Clinical Relevance:
CPDA-1 blood collection bags stored at either 24 °C or 5 °C can be used in a multi-dose manner for up to 20 days when each sample is obtained aseptically. These results support the clinician's ability to utilize the contents of 1 bag multiple times rather than discarding the bag after a single use.
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