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Updated: Aug 2, 2025
LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism
Published on: April 4, 2016
LC-MS/MS method for the quantitation of OCT1 biomarker isobutyrylcarnitine in human plasma with clinical sample
Amy Troop1, Eugene Kadar1, Emi Kimoto1
1Pharmacokinetics, Dynamics & Metabolism, Pfizer Global Research, Eastern Point Road, Groton, CT 06340, USA.
Abstract:
Aim: Isobutyrylcarnitine (IBC) is a possible biomarker for hepatic OCT1, as IBC plasma concentrations are reduced when OCT1 is inhibited. An accessible, characterized assay is needed to quantitate IBC in human plasma. Materials & methods: A triple quadrupole MS surrogate matrix assay for the quantitation of IBC was characterized to support a first-in-human study. Results: An assay for IBC quantitation was fully characterized for accuracy, precision, selectivity and parallelism. IBC was measured in a clinical study and the data were correlated to the in vitro model prediction. Conclusion: A triple quadrupole-based assay for IBC should broaden the monitoring of IBC for OCT1 inhibition in early clinical trials, generating the data needed to establish IBC as a valid biomarker.
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