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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
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Confocal Fluorescence Microscopy01:16

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry
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Development of Spectral Imaging Cytometry.

Ivan A Vorobjev1,2,3,4, Aigul Kussanova5,6, Natasha S Barteneva5,7

  • 1School of Sciences and Humanities, Nazarbayev University, Astana, Kazakhstan. ivan.vorobyev@nu.edu.kz.

Methods in Molecular Biology (Clifton, N.J.)
|April 19, 2023
PubMed
Summary

Spectral flow cytometry precisely measures cellular properties using advanced technology. This review covers its history, instrument comparison, and diverse applications, including analyzing autofluorescence in various cells.

Keywords:
Aurora cytometerAutofluorescenceFlow cytometryFluorescence spectraSony spectral analyzerSpectral cytometrySpectral unmixingVirtual filtering

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Area of Science:

  • Cellular and Molecular Biology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Spectral flow cytometry is an advanced technique for high-precision cellular analysis.
  • Modern instruments offer multi-parameter measurements, distinguishing overlapping fluorescent signals and autofluorescence.

Approach:

  • This paper reviews the historical development of spectral flow cytometry.
  • It compares modern spectral flow cytometers with conventional ones.
  • The review discusses various applications of this technology.

Key Points:

  • Enables simultaneous determination of 40+ fluorescent dyes with overlapping spectra.
  • Facilitates discrimination of autofluorescent signals in diverse cell types.
  • Provides detailed analysis of autofluorescence from mammalian to cyanobacteria cells.

Conclusions:

  • Spectral flow cytometry represents a significant advancement in cellular analysis.
  • Its capabilities extend to complex biological samples, including microorganisms.
  • The technology offers broad applications across scientific research fields.