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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Aug 2, 2025

Molecular Probe Optimization to Determine Cell Mortality in a Photosynthetic Organism Microcystis aeruginosa Using Flow Cytometry

Molecular Probe Optimization to Determine Cell Mortality in a Photosynthetic Organism Microcystis aeruginosa Using Flow Cytometry

Published on: January 29, 2016

Optimizing FlowCam Imaging Flow Cytometry Operation for Classification and Quantification of Microcystis

Dmitry Malashenkov¹, Veronika Dashkova², Ivan A Vorobjev^1,3

¹Department of Biology, School of Sciences and Humanities, Nazarbayev University, Astana, Kazakhstan.

Methods in Molecular Biology (Clifton, N.J.)

|April 19, 2023

Summary

Quantifying Microcystis blooms is challenging. Imaging flow cytometry (IFC) with FlowCam offers an objective method to identify and count different Microcystis species in environmental samples.

Keywords:

CyanoHABs Cyanobacteria FlowCam Imaging flow cytometry Microcystis Phytoplankton

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Characterization of Aquatic Biofilms with Flow Cytometry

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Last Updated: Aug 2, 2025

Molecular Probe Optimization to Determine Cell Mortality in a Photosynthetic Organism Microcystis aeruginosa Using Flow Cytometry

Molecular Probe Optimization to Determine Cell Mortality in a Photosynthetic Organism Microcystis aeruginosa Using Flow Cytometry

Published on: January 29, 2016

Author Spotlight: Advancing Research in Microbial Autoaggregation Using Imaging Flow Cytometry

Author Spotlight: Advancing Research in Microbial Autoaggregation Using Imaging Flow Cytometry

Published on: September 29, 2023

Characterization of Aquatic Biofilms with Flow Cytometry

Characterization of Aquatic Biofilms with Flow Cytometry

Published on: June 6, 2018

Area of Science:

Environmental microbiology
Aquatic ecology
Phycology

Background:

Microcystis cyanobacteria cause toxic blooms globally.
Distinguishing Microcystis morphospecies is crucial but difficult with light microscopy.
Current methods for Microcystis quantification are often time-consuming and imprecise.

Purpose of the Study:

To present a FlowCam methodology for Microcystis morphospecies identification and quantification.
To analyze five common European Microcystis morphospecies using imaging flow cytometry.
To provide objective data for Microcystis bloom analysis.

Main Methods:

Utilized a benchtop imaging flow cytometer (FlowCam).
Developed sample processing and analysis protocols for environmental samples.
Focused on five European Microcystis morphospecies found in temperate zones.

Main Results:

The FlowCam technique successfully detected and differentiated Microcystis morphospecies.
Objective qualitative and quantitative data were generated.
The method facilitates statistical analysis of Microcystis populations.

Conclusions:

Imaging flow cytometry provides an efficient and objective approach for Microcystis analysis.
FlowCam methodology enables accurate quantification of co-occurring Microcystis morphospecies.
This technique supports better understanding and management of Microcystis blooms.