Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stress Fractures in a U.S. Military Population by Duration of Active Duty-A Retrospective, Sex-Stratified Cross-Sectional Study.

Military medicine·2026
Same author

Forgiveness and mental health in military personnel with chronic orofacial pain.

Journal of health psychology·2026
Same author

Does Race Affect Utilization of Unicompartmental vs Total Knee Arthroplasty? A Matched Cohort Study Within a Universal Health System.

Arthroplasty today·2026
Same author

Predictors of Respiratory Oscillometry Measurements in a Healthy Population.

Respiratory care·2025
Same author

Impact of tongue fat volume on obstructive sleep apnea in non-obese patients.

Scientific reports·2025
Same author

Use of the 36-Point Thyroid Symptom Questionnaire to Potentially Guide Optimal Thyroid Hormone Replacement Therapy.

Endocrine practice : official journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists·2025

Related Experiment Video

Updated: Aug 2, 2025

Basophil Activation Test for Allergy Diagnosis
07:22

Basophil Activation Test for Allergy Diagnosis

Published on: May 31, 2021

8.3K

Hymenoptera venom skin testing: Adopting an accelerated test protocol.

Qing Wang1, Nora L Watson2, Douglas E Beakes1

  • 1Allergy and Immunology, Walter Reed National Military Medical Center, Bethesda, Maryland.

Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology
|April 19, 2023
PubMed
Summary
This summary is machine-generated.

Accelerated venom skin testing is safe and effective, with no adverse reactions observed in patients. This method identifies positive results at higher concentrations, reducing testing time and costs.

More Related Videos

Protocols for Testing the Toxicity of Novel Insecticidal Chemistries to Mosquitoes
09:32

Protocols for Testing the Toxicity of Novel Insecticidal Chemistries to Mosquitoes

Published on: February 13, 2019

15.7K
An In Vitro Skin Irritation Test SIT using the EpiDerm Reconstructed Human Epidermal RHE Model
21:16

An In Vitro Skin Irritation Test SIT using the EpiDerm Reconstructed Human Epidermal RHE Model

Published on: July 13, 2009

68.0K

Related Experiment Videos

Last Updated: Aug 2, 2025

Basophil Activation Test for Allergy Diagnosis
07:22

Basophil Activation Test for Allergy Diagnosis

Published on: May 31, 2021

8.3K
Protocols for Testing the Toxicity of Novel Insecticidal Chemistries to Mosquitoes
09:32

Protocols for Testing the Toxicity of Novel Insecticidal Chemistries to Mosquitoes

Published on: February 13, 2019

15.7K
An In Vitro Skin Irritation Test SIT using the EpiDerm Reconstructed Human Epidermal RHE Model
21:16

An In Vitro Skin Irritation Test SIT using the EpiDerm Reconstructed Human Epidermal RHE Model

Published on: July 13, 2009

68.0K

Area of Science:

  • Allergy and Immunology
  • Clinical Research
  • Dermatology

Background:

  • Standard Hymenoptera venom intradermal skin testing starts at low concentrations (0.001–0.01 μg/mL), increasing 10-fold to a maximum of 1 μg/mL.
  • Accelerated venom skin test protocols, starting at higher concentrations, are reported as safe but are not widely adopted.
  • This study evaluates the safety and efficacy of accelerated venom skin testing.

Purpose of the Study:

  • To compare the outcomes and safety of standard versus accelerated venom skin test protocols.
  • To determine the optimal concentration ranges for positive venom allergy identification.

Main Methods:

  • A retrospective chart review of 211 patients (134 standard, 77 accelerated) with suspected venom allergy from 2012–2022.
  • Analysis of demographic data, skin test protocols, results, and adverse reactions.
  • Comparison of adverse event rates and positive test result concentrations between standard and accelerated protocols.

Main Results:

  • No adverse reactions were reported in the 77 patients undergoing accelerated venom skin testing.
  • Two adverse reactions (1.5%) occurred in 134 patients undergoing standard testing: one urticaria and one anaphylaxis.
  • Over 75% of positive results in the standard protocol and over 60% in the accelerated protocol occurred at 0.1 or 1 μg/mL.

Conclusions:

  • Venom intradermal skin testing is generally safe.
  • Accelerated protocols are safe and identify positive results at higher concentrations (0.1–1 μg/mL).
  • Adopting accelerated venom skin testing can reduce testing time and healthcare costs.