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Updated: Aug 2, 2025

Measuring Skeletal Muscle Thermogenesis in Mice and Rats

Measuring Skeletal Muscle Thermogenesis in Mice and Rats

Published on: July 27, 2022

Thermogenic Phenotyping in Mice.

Donghua Hu¹, Irfan J Lodhi^2,3

¹Division of Endocrinology, Metabolism and Lipid Research, Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA. hudonghua@wustl.edu.

Methods in Molecular Biology (Clifton, N.J.)

|April 19, 2023

Summary

This summary is machine-generated.

Accurately measure brown fat activation in mice using implantable temperature transponders and indirect calorimetry. These methods assess thermogenic phenotypes, crucial for understanding metabolic homeostasis and energy expenditure in preclinical studies.

Keywords:

Body temperature Energy expenditure Indirect calorimetry Metabolic cages Oxygen consumption Thermogenesis

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Area of Science:

Metabolic Physiology
Adipose Tissue Biology
Preclinical Research Methods

Background:

Brown adipose tissue (BAT) thermogenesis is vital for metabolic homeostasis in mammals.
Quantifying thermogenic responses is essential for characterizing metabolic phenotypes.
Preclinical models require precise methods to assess brown fat activation.

Purpose of the Study:

To describe two methods for assessing thermogenic phenotypes in mice.
To enable accurate measurement of metabolic responses to brown fat activation.
To characterize thermogenic phenotypes under non-basal conditions.

Main Methods:

Implantable temperature transponders for continuous body temperature monitoring in cold-exposed mice.
Indirect calorimetry to measure oxygen consumption changes.
Assessment of β3-adrenergic agonist-stimulated thermogenesis.

Main Results:

Continuous body temperature monitoring provides detailed thermogenic responses.
Indirect calorimetry quantifies metabolic rate changes indicative of BAT activation.
The described methods allow for comprehensive assessment of thermogenic capacity.

Conclusions:

These methods offer robust tools for evaluating thermogenic phenotypes in mice.
Accurate assessment of brown fat function is critical for metabolic research.
The protocols facilitate preclinical studies on metabolic regulation and energy balance.