Dyadobacter pollutisoli sp. nov., isolated from plastic waste landfill soil

A Gram-stain-negative, yellow-pigmented and facultatively aerobic bacterium, designated strain U1^T, was isolated from plastic dumped soil sampled in the Republic of Korea. Cell of strain U1^T were non-motile rods showing catalase-negative and oxidase-positive activities. Strain U1^T was shown to grow at 10-37 °C (optimum, 25-30 °C) and pH 6.0-9.0 (optimum, pH 8.0), and in the presence of 0-0.5 % (w/v) NaCl (optimum, 0 %). Strain U1^T contained iso-C_15 : 0, C_16 : 0, C_16 : 1 ω5c and summed feature 3 (comprising C_16 : 1  ω6c and/or C_16 : 1 ω7c) as the major cellular fatty acids (>5 %) and menaquinone-7 as the sole respiratory quinone. Phosphatidylethanolamine, two unidentified aminolipids and three unidentified lipids were identified as the major polar lipids. The DNA G+C content of strain U1^T calculated from the whole-genome sequence was 45.5 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain U1^T formed a distinct phylogenetic lineage within the genus Dyadobacter. Strain U1^T has the highest 16S rRNA sequence similarity to Dyadobacter bucti QTA69^T (97.9 %). Average nucleotide identity and digital DNA-DNA hybridization values between strain U1^T and D. bucti QTA69^T were 74.6 % and 18.9 %, respectively. Based on phenotypic, chemotaxonomic and molecular features, strain U1^T represents a novel species of the genus Dyadobacter, for which the name Dyadobacter pollutisoli sp. nov. is proposed. The type strain is U1^T (= KACC 22210^T=JCM 34491^T).