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Tracking of Intestinal Probiotics In Vivo by NIR-IIb Fluorescence Imaging.

Chenhui Ji1, Yao Huang1, Leilei Sun1

  • 1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, Hunan, China.

ACS Applied Materials & Interfaces
|April 20, 2023
PubMed
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This summary is machine-generated.

Researchers developed a novel method to track bacteria in the gut using near-infrared-IIb (NIR-IIb) lanthanide nanomaterials. This technique improves visualization of microbial distribution in intestinal tissues.

Area of Science:

  • Biomedical Engineering
  • Microbiology
  • Nanotechnology

Background:

  • Accurate characterization of microorganism distribution in the intestinal tract is crucial for understanding biological mechanisms.
  • Traditional optical probes for microbial labeling in the intestine have limitations in imaging penetration depth and resolution.

Purpose of the Study:

  • To develop a novel observation tool for microbial research using advanced nanomaterials.
  • To overcome the limitations of traditional imaging techniques for in vivo microbial tracking.

Main Methods:

  • Labeling of *Lactobacillus bulgaricus* (*L. bulgaricus*) with NaGdF₄:Yb³⁺,Er³⁺@NaGdF₄,Nd³⁺ (Er@Nd NPs) near-infrared-IIb (NIR-IIb) lanthanide nanomaterials via EDC-NHS chemistry.
  • Monitoring labeled microorganisms in tissue using two-photon excitation (TPE) microscopy.
Keywords:
intestinal transplanted bacteriamicroorganism monitoringmicroorganism trackingnear-infrared-IIb imagingnear-infrared-IIb lanthanide nanomaterials

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  • In vivo imaging of microbial distribution using NIR-IIb imaging.

    • Main Results:

    • Successful labeling of *L. bulgaricus* with Er@Nd NPs.
    • Demonstration of dual-technique imaging (TPE microscopy and NIR-IIb imaging) for microorganism monitoring.
    • Enhanced spatiotemporal resolution for observing microbial distribution in the intestinal tract.

    Conclusions:

    • The developed NIR-IIb lanthanide nanomaterial labeling provides a powerful tool for microbial research.
    • This dual-imaging approach significantly improves the ability to determine the distribution of transplanted bacteria in the intestinal tract.
    • The method offers great potential for advancing our understanding of gut microbiome dynamics and therapeutic interventions.