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GTSE1 promotes the growth of NSCLC by regulating microtubule-associated proteins through the ERK/MAPK pathway

Chuanlin Wang1,2, Meiyan Wen1,2, Jiali Xu1,2

  • 1Department of Clinical Nutrition, Yunnan Cancer Hospital/The Third Affiliated Hospital of Kunming Medical University, Kunming, China.

Thoracic Cancer
|April 20, 2023
PubMed

Insights

G2 and S phase-expressed-1 (GTSE1) promotes non-small-cell lung cancer (NSCLC) growth by affecting microtubules. Upregulated GTSE1 correlates with metastasis and shorter survival, suggesting it as a potential therapeutic target.

Area of Science:

  • Oncology
  • Cell Biology
  • Molecular Biology

Background:

  • The role of G2 and S phase-expressed-1 (GTSE1), a microtubule-associated protein, in non-small-cell lung cancer (NSCLC) pathogenesis is not well understood.
  • GTSE1's function in cancer progression warrants investigation for potential therapeutic strategies.

Purpose of the Study:

  • To investigate the role and clinical significance of GTSE1 in non-small-cell lung cancer (NSCLC) growth and progression.
  • To elucidate the molecular mechanisms by which GTSE1 influences NSCLC cell behavior and microtubule dynamics.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) to assess GTSE1 expression in NSCLC tissues and cell lines.
  • Transwell, cell-scratch, MTT assays, flow cytometry, and western blotting to evaluate biological and apoptotic effects.
  • Immunofluorescence and western blotting to confirm GTSE1's association with cellular microtubules.

Main Results:

  • GTSE1 expression was significantly upregulated in NSCLC tissues and cell lines compared to normal controls.
  • Higher GTSE1 mRNA levels correlated with lymph node metastasis and shorter progression-free survival in NSCLC patients.
  • GTSE1 knockdown suppressed NSCLC cell proliferation, colony formation, invasion, and migration, and inhibited microtubule-associated proteins tau and stathmin-1 via the ERK/MAPK pathway.

Conclusions:

  • GTSE1 is upregulated in NSCLC and associated with adverse clinical outcomes, indicating its oncogenic potential.
  • GTSE1 promotes NSCLC growth by modulating tau and stathmin-1 expression through the extracellular-regulated protein kinase/mitogen-activated protein kinase (ERK/MAPK) signaling pathway, leading to microtubule disruption.
  • GTSE1 represents a potential therapeutic target for managing non-small-cell lung cancer.
Keywords:
ERK/MAPK pathwayGTSE1microtubulenon-small-cell lung cancer

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