Development of composite film based on collagen and phenolic acid-grafted chitosan for food packaging

Collagen, a fibrous protein with triple-helical structure, is a good film-forming substrate for food packaging films because collagen films show advantages of biodegradability, high mechanical strength and good water resistance. However, collagen films lack functional activities, which may limit their applications in the field of active packaging. In this work, phenolic acid-grafted-chitosan was blended with collagen to improve the antioxidant and antimicrobial activities of collagen films. Gallic acid (GA), ferulic acid (FA) and caffeic acid (CA) were respectively grafted onto chitosan, and the physical properties and functional activities of the collagen/phenolic acids-g-chitosan (CGC, CFC and CCC) films were compared. The prepared films presented varying degrees of yellow color, and exhibited significantly improved UV light blocking capacity, antioxidant and antimicrobial properties due to the function of phenolic acid. Moreover, compared with collagen/chitosan (CC) film, CGC, CFC and CCC films showed higher mechanical strength (69.08-73.79 MPa), higher thermal denaturation temperature (69.4-71.2 °C), and lower water vapor permeability values (2.64-2.98 × 10^-12 g m^-1 s^-1 Pa^-1). The properties of collagen/ phenolic acids-g-chitosan films were greatly affected by the type of phenolic acid grafted. CGC film had the best antioxidant property as well as the best mechanical property, thermostability, UV light and water vapor blocking capacity.