Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Aug 2, 2025

Author Spotlight: Advancing Lung Transplant Immunology Through Intravital Imaging
04:16

Author Spotlight: Advancing Lung Transplant Immunology Through Intravital Imaging

Published on: April 19, 2024

1.1K

Single-cell transcriptomics of a dynamic cell behavior in murine airways.

Sheldon J J Kwok1,2, Daniel T Montoro3,4, Adam L Haber5

  • 1Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, Cambridge, United States.

Elife
|April 21, 2023
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Shifting IRES versus Cap-initiated translation during homeostatic stem cell differentiation and stress.

Science advances·2026
Same author

Air-Stable Room-Temperature Quasi-2D Tin Iodide Perovskite Microlasers.

ACS photonics·2026
Same author

Discovering paracrine regulators of cell type composition from spatial transcriptomics using SPER.

Bioinformatics advances·2026
Same author

Hic-5 drives epithelial mechanotransduction promoting a feed-forward cycle of bronchoconstriction.

Nature communications·2025
Same author

A molecular circuit regulates fate plasticity in emerging and adult AT2 cells.

Nature communications·2025
Same author

Single-cell analysis of human airway epithelium identifies cell-type-specific responses to <i>Aspergillus</i> and <i>Coccidioides</i>.

mBio·2025

We developed a new method to link cell behavior in tissues to molecular profiles. This approach identified a unique molecular signature for cell migration after airway injury in mice.

Area of Science:

  • Cellular Biology
  • Molecular Biology
  • Tissue Engineering

Background:

  • High-dimensional cellular analysis has advanced, but profiling dynamic cell behaviors in native environments is challenging.
  • Understanding cell migration and molecular responses in intact tissues is crucial for regenerative medicine and disease research.

Purpose of the Study:

  • To develop and validate a novel method for coupling physiological cell behaviors in intact tissues with deep molecular profiling.
  • To identify molecular signatures associated with specific cellular dynamics, such as migration following injury.

Main Methods:

  • Integration of live-cell imaging techniques with single-cell molecular profiling (e.g., transcriptomics, proteomics).
  • Application of the method to analyze cellular responses within an intact murine airway tissue model following induced injury.
Keywords:
airwaysdevelopmental biologymigrationmouseregenerative medicinestem cellstwo-photon imaging

More Related Videos

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes
08:23

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

Published on: December 28, 2011

17.5K
Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.6K

Related Experiment Videos

Last Updated: Aug 2, 2025

Author Spotlight: Advancing Lung Transplant Immunology Through Intravital Imaging
04:16

Author Spotlight: Advancing Lung Transplant Immunology Through Intravital Imaging

Published on: April 19, 2024

1.1K
Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes
08:23

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

Published on: December 28, 2011

17.5K
Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.6K
  • Development of computational tools to correlate observed cell behaviors with molecular data.

    • Main Results:

    • Successfully coupled real-time cellular physiological behaviors in intact murine airways to deep molecular profiling of individual cells.
    • Identified a novel molecular signature associated with a striking migratory cellular behavior observed after airway injury.
    • Demonstrated the feasibility of analyzing dynamic cellular processes and their molecular underpinnings in a native tissue context.

    Conclusions:

    • The presented method overcomes limitations in studying dynamic cellular processes in vivo.
    • The identified molecular signature provides new insights into the mechanisms of cell migration post-injury.
    • This approach has broad applications for molecular profiling of cellular dynamics in various biological contexts and diseases.