Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Micronutrients and midnight: the diet-sleep link.

Sleep·2025

Same author

4D mitochondrial network assumes distinct and predictive phenotypes through human lung and intestinal epithelial development.

bioRxiv : the preprint server for biology·2025

Same author

Rapid iPSC inclusionopathy models shed light on formation, consequence, and molecular subtype of α-synuclein inclusions.

Neuron·2025

Same author

LiveLattice: Real-time visualisation of tilted light-sheet microscopy data using a memory-efficient transformation algorithm.

Journal of microscopy·2024

Same author

Rapid iPSC inclusionopathy models shed light on formation, consequence, and molecular subtype of α-synuclein inclusions.

Neuron·2024

Same author

LiveLattice: Real-time visualization of tilted light-sheet microscopy data using a memory-efficient transformation algorithm.

bioRxiv : the preprint server for biology·2024

Search research articles

Related Experiment Video

Updated: Aug 2, 2025

A Faster, High Resolution, mtPA-GFP-based Mitochondrial Fusion Assay Acquiring Kinetic Data of Multiple Cells in Parallel Using Confocal Microscopy

A Faster, High Resolution, mtPA-GFP-based Mitochondrial Fusion Assay Acquiring Kinetic Data of Multiple Cells in Parallel Using Confocal Microscopy

Published on: July 20, 2012

MitoTNT: Mitochondrial Temporal Network Tracking for 4D live-cell fluorescence microscopy data.

Zichen Wang^1,2, Parth Natekar^1,2, Challana Tea^1,2

¹Department of Pharmacology, University of California, San Diego, San Diego, California, United States of America.

Plos Computational Biology

|April 21, 2023

Summary

This summary is machine-generated.

MitoTNT software tracks mitochondrial networks in 4D live-cell microscopy, enabling accurate analysis of mitochondrial dynamics and disease implications. This tool enhances understanding of cellular processes and potential therapeutic targets.

More Related Videos

Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy

Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy

Published on: June 4, 2020

Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations

Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations

Published on: June 17, 2011

Related Experiment Videos

Last Updated: Aug 2, 2025

A Faster, High Resolution, mtPA-GFP-based Mitochondrial Fusion Assay Acquiring Kinetic Data of Multiple Cells in Parallel Using Confocal Microscopy

A Faster, High Resolution, mtPA-GFP-based Mitochondrial Fusion Assay Acquiring Kinetic Data of Multiple Cells in Parallel Using Confocal Microscopy

Published on: July 20, 2012

Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy

Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy

Published on: June 4, 2020

Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations

Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations

Published on: June 17, 2011

Area of Science:

Cell Biology
Mitochondrial Dynamics
Bioimaging

Background:

Mitochondria form a dynamic 4D network crucial for cellular health.
Dysregulation of mitochondrial networks is linked to diseases like cancer and neurodegeneration.
Existing 4D imaging methods lack quantitative analysis tools for mitochondrial networks.

Purpose of the Study:

Introduce MitoTNT, the first software for Mitochondrial Temporal Network Tracking (MitoTNT) in 4D live-cell microscopy.
Provide accurate quantitative analysis of mitochondrial network dynamics.
Facilitate research into mitochondrial roles in health and disease.

Main Methods:

Developed MitoTNT software utilizing spatial proximity and network topology for optimal tracking.
Validated tracking accuracy using reaction-diffusion simulations and experimental data.
Applied MitoTNT to quantify 4D mitochondrial networks in human induced pluripotent stem cells.

Main Results:

MitoTNT achieved >90% accuracy in simulations and validated well with experimental data.
Characterized mitochondrial skeleton node motion, revealing temporal correlations.
Identified fission/fusion events, showing increased node motility near these sites and a role for microtubules.
Transport simulations revealed that pharmacological perturbations affect network reachability and resilience.

Conclusions:

MitoTNT provides accurate, accessible 4D mitochondrial network tracking and analysis.
Revealed novel insights into mitochondrial dynamics, including node motion patterns and fission/fusion mechanisms.
Demonstrated the impact of perturbations on mitochondrial network function, relevant for disease and drug development.