Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inhibition of Mincle signaling by chemically synthesized disaccharide-type 6-<i>O</i>-acylated steryl β-glucosides (βASGs) and their analogues derived from plants.

Chemical communications (Cambridge, England)·2026
Same author

Recent trends in therapeutic ultrasound: focusing on technologies that utilize non-thermal effects.

Journal of medical ultrasonics (2001)·2026
Same author

Subretinal injection in the USH1CR31∗ pig model leads to chorioretinal atrophy that limits evaluation of efficacy of an AAV-mediated gene therapy.

Experimental eye research·2026
Same author

Treatment of spinal injury muscle spasticity by spinal subpial AAV9-GAD65/VGAT delivery: An efficacy and safety study in rat, pig, and NHP.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

Molecular features of a Huntington's disease knock-in minipig.

Disease models & mechanisms·2026
Same author

Long-term safety and clinical outcomes from a single-site phase 1 study of neural stem cell transplantation for chronic cervical spinal cord injury.

Stem cell reports·2026

Related Experiment Video

Updated: Aug 1, 2025

Promotion of Survival and Differentiation of Neural Stem Cells with Fibrin and Growth Factor Cocktails after Severe Spinal Cord Injury
09:56

Promotion of Survival and Differentiation of Neural Stem Cells with Fibrin and Growth Factor Cocktails after Severe Spinal Cord Injury

Published on: July 27, 2014

12.5K

Expandable Sendai-Virus-Reprogrammed Human iPSC-Neuronal Precursors: In Vivo Post-Grafting Safety Characterization in

Yoshiomi Kobayashi1,2, Michiko Shigyo1, Oleksandr Platoshyn1

  • 1Department of Anesthesiology, School of Medicine, University of California, San Diego, San Diego, CA, USA.

Cell Transplantation
|April 24, 2023
PubMed
Summary

Previously frozen human neural precursor cells (hiPSC-NPCs) demonstrate effective engraftment, comparable to fresh cells. A novel human spinal injection device also proved safe for cell therapy delivery.

Keywords:
cryopreservationhuman induced pluripotent stem cells (hiPSCs)human injection deviceimmunosuppressed adult pigneural precursor cells (NPCs)spinal cord

More Related Videos

Conversion of Human Induced Pluripotent Stem Cells iPSCs into Functional Spinal and Cranial Motor Neurons Using PiggyBac Vectors
07:33

Conversion of Human Induced Pluripotent Stem Cells iPSCs into Functional Spinal and Cranial Motor Neurons Using PiggyBac Vectors

Published on: May 1, 2019

11.5K
Intracerebral Transplantation and In Vivo Bioluminescence Tracking of Human Neural Progenitor Cells in the Mouse Brain
06:12

Intracerebral Transplantation and In Vivo Bioluminescence Tracking of Human Neural Progenitor Cells in the Mouse Brain

Published on: January 27, 2022

3.2K

Related Experiment Videos

Last Updated: Aug 1, 2025

Promotion of Survival and Differentiation of Neural Stem Cells with Fibrin and Growth Factor Cocktails after Severe Spinal Cord Injury
09:56

Promotion of Survival and Differentiation of Neural Stem Cells with Fibrin and Growth Factor Cocktails after Severe Spinal Cord Injury

Published on: July 27, 2014

12.5K
Conversion of Human Induced Pluripotent Stem Cells iPSCs into Functional Spinal and Cranial Motor Neurons Using PiggyBac Vectors
07:33

Conversion of Human Induced Pluripotent Stem Cells iPSCs into Functional Spinal and Cranial Motor Neurons Using PiggyBac Vectors

Published on: May 1, 2019

11.5K
Intracerebral Transplantation and In Vivo Bioluminescence Tracking of Human Neural Progenitor Cells in the Mouse Brain
06:12

Intracerebral Transplantation and In Vivo Bioluminescence Tracking of Human Neural Progenitor Cells in the Mouse Brain

Published on: January 27, 2022

3.2K

Area of Science:

  • Regenerative Medicine
  • Neuroscience
  • Biotechnology

Background:

  • Clinical translation of cell-replacement therapies faces challenges in cell preparation and delivery.
  • Optimal protocols for cell generation, storage, and recovery are needed for patient administration.
  • Simple, safe injection devices are crucial for widespread adoption of spinal cell therapies.

Purpose of the Study:

  • To compare the engraftment of fresh versus frozen human-induced pluripotent stem cells (hiPSCs)-derived neural precursor cells (NPCs).
  • To evaluate the safety of a novel human spinal injection device for cell delivery.

Main Methods:

  • Human-induced pluripotent stem cells (hiPSCs)-derived neural precursor cells (NPCs) were either used fresh or after freezing.
  • Cells were grafted into the striata or spinal cord of immunodeficient rats.
  • A new human spinal injection device was tested in adult immunosuppressed pigs.

Main Results:

  • Previously frozen NPCs exhibited comparable post-grafting survival and differentiation to freshly harvested cells.
  • The human injection device demonstrated acceptable safety, with no procedure-related side effects observed in pigs.

Conclusions:

  • Cryopreservation of hiPSC-derived NPCs is a viable option for cell-replacement therapies.
  • The tested human spinal injection device is safe and suitable for clinical application, facilitating potential future dissemination of spinal cell therapies.