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Updated: Aug 1, 2025

Using Cyclic Voltammetry, UV-Vis-NIR, and EPR Spectroelectrochemistry to Analyze Organic Compounds
Published on: October 18, 2018
Novel Experimental and Analysis Strategies for Fast Voltammetry: 2. A Troubleshoot-Free Flow Cell for FSCV
Melissa Hexter1, Joseph van Batenburg-Sherwood1, Parastoo Hashemi1
1Department of Bioengineering, Imperial College London, SW7 2AZLondon, U.K.
Abstract:
Fast scan cyclic voltammetry (FSCV) at carbon fiber microelectrodes (CFMs) is a method traditionally used for real-time quantification of neurotransmitters in biological systems. Reliable calibration of CFMs is essential for converting FSCV signals to analyte concentrations and generally employs flow injection analysis (FIA) performed with flow cells fabricated in-house. Such FSCV FIA cells often require significant and ongoing troubleshooting with pulsing, leaking, flow inconsistencies and dead volume being major causes of common challenges. In this work, we address these issues by creating a robust, plug-and-play FSCV flow cell. This novel design permits reproducible, high-precision, and stable flow injection profiles using low-cost materials to improve FSCV calibration. The ready-to-print computer-aided designs and hardware list are provided.
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