Validation of proteins associated with pathological damage in human tuberculosis granulomas: study protocol
Thabo Mpotje1,2, Jessica More1,2, Kerishka Rajkumar-Bhugeloo1,2
1School of Laboratory Medicine and Medical Sciences, University of KwaZulu-Natal, Durban, KwaZulu-Natal, 4001, South Africa.
Wellcome Open Research
|April 24, 2023
Summary
Tuberculosis (TB) causes lung damage via granuloma breakdown. This study identifies host factors driving this pathology to understand TB-induced immunopathology and lung destruction.
Area of Science:
- Immunopathology
- Pulmonology
- Microbiology
Background:
- Tuberculosis (TB) is caused by Mycobacterium Tuberculosis (Mtb).
- Mtb infection leads to granuloma formation to contain bacteria, but granuloma dissociation causes lung caseation and destruction.
- Understanding TB-induced immunopathology is crucial due to the growing global burden of lung function challenges.
Purpose of the Study:
- To explore host factors driving pathological features of TB.
- To investigate mechanisms contributing to extensive lung tissue destruction in TB patients.
Main Methods:
- Analysis of lung tissue sections from patients undergoing surgical resection.
- Histopathological assays including Immunohistochemistry, Immunofluorescence, and Hematoxylin and Eosin staining.
- Laser Capture Microdissection for detailed analysis.
Main Results:
- Identification of key host factors associated with TB immunopathology.
- Detailed characterization of pathological features contributing to lung destruction.
Conclusions:
- Key host factors driving exacerbated lung immunopathology in TB have been identified.
- Findings contribute to a deeper understanding of TB pathogenesis and lung tissue destruction.
Related Concept Videos
01:26Pulmonary Tuberculosis IV
184
Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
184
01:28Pulmonary Tuberculosis II
283
Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
283
01:31Pulmonary Tuberculosis III
387
Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
The first classification is based on the development of the disease, and it includes the following categories:
The first classification is based on the development of the disease, and it includes the following categories:
387
01:29Pulmonary Tuberculosis I
294
Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
294
01:28Pulmonary Tuberculosis V
219
Medical management of tuberculosis (TB) patients involves a comprehensive approach that includes diagnosis, treatment, and monitoring. The specific strategies can vary depending on the type of tuberculosis (latent or active), the patient's overall health status, and other considerations.
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the...
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the...
219