Coronavirus genomic cDNA derived G-quadruplex as a selective target for fluorometric detection

Area of Science:

• Genomics
• Molecular Biology
• Diagnostic Development

Background:

• Pathogenic genomes can contain noncanonical G-quadruplex (GQ) forming sequences.
• These GQ-forming sequences represent potential targets for disease diagnosis.

Purpose of the Study:

• To identify and validate G-quadruplex (GQ) forming cDNA sequences within the SARS-CoV-2 (Severe acute respiratory syndrome coronavirus-2) genome.
• To demonstrate the utility of these sequences as reliable diagnostic targets.

Main Methods:

• Bioinformatic identification of potential GQ-forming sequences in the SARS-CoV-2 genome.
• Experimental validation of identified cDNA GQs.
• Development and application of small molecular probes for fluorescence detection.

Main Results:

• GQ-forming cDNA sequences specific to SARS-CoV-2 were identified and validated.
• High-fidelity fluorescence detection of these SARS-CoV-2 derived cDNA GQs was achieved.
• Demonstrated the potential of these probes for accurate detection.

Conclusions:

• SARS-CoV-2 cDNA GQs are validated as reliable targets for molecular diagnostics.
• Small molecular probes offer a sensitive and specific method for detecting these diagnostic targets.
• This approach holds promise for the development of novel diagnostic tools for SARS-CoV-2.