Related Experiment Video
Updated: Aug 1, 2025

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
Published on: April 4, 2025
Coronavirus genomic cDNA derived G-quadruplex as a selective target for fluorometric detection
Sumon Pratihar1, Mohamed Nabeel Mattath1, Thimmaiah Govindaraju1
1Bioorganic Chemistry Laboratory, New Chemistry Unit, Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR), Jakkur, P.O., Bengaluru, 560064, Karnataka, India. tgraju@jncasr.ac.in.
Abstract:
Pathogenic genomes harboring noncanonical G-quadruplex (GQ) forming sequences are potential targets for diagnosis. The GQ-forming cDNA sequences of SARS-CoV-2 (Severe acute respiratory syndrome coronavirus-2) are identified and validated as reliable diagnostic targets. The high fidelity fluorescence detection of specific cDNA GQs derived from the SARS-CoV-2 RNA genome is demonstrated using small molecular probes.

