Related Experiment Video
Updated: Apr 10, 2026
07:23
One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster
Published on: January 26, 2024
1.7K
Traceable gene delivery using minimalistic far-red fluorescent pseudodendrimers.
Hariharan Moorthy1, Pardhasaradhi Satha1, Madhu Ramesh1
1Bioorganic Chemistry Laboratory, New Chemistry Unit, Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR), Bengaluru 560064, Karnataka, India. tgraju@jncasr.ac.in.
Chemical Communications (Cambridge, England)
|April 9, 2026
Summary
Researchers developed a novel far-red fluorescent pseudodendrimer (PD) for efficient gene delivery. This platform enables fluorescence tracking and high transfection efficiency, outperforming existing methods.
Area of Science:
- Biomaterials Science
- Gene Therapy
- Nanotechnology
Background:
- Gene delivery systems are crucial for nucleic acid therapeutics.
- Current methods face challenges in efficiency, traceability, and biocompatibility.
- Development of advanced delivery platforms is essential for next-generation theranostics.
Purpose of the Study:
- To develop a minimalistic, intrinsically far-red fluorescent pseudodendrimer (PD) platform for efficient and traceable gene delivery.
- To evaluate the performance of arginine-functionalized PD (TGP 41) in condensing DNA and forming stable nanocondensates (NCs).
- To compare the transfection efficiency and traceability of TGP 41 NCs with a commercial transfection agent.
Main Methods:
- Synthesis of an arginine-functionalized pseudodendrimer (TGP 41).
- Formation of DNA nanocondensates (NCs) using TGP 41 and low Ca2+ concentrations.
- Assessment of NC stability, DNA condensation, and transfection efficiency.
- Evaluation of fluorescence traceability using the intrinsic far-red fluorescence of the PD.
Main Results:
- TGP 41 efficiently condensed DNA into stable nanocondensates (NCs).
- TGP 41 NCs demonstrated high transfection efficiency, comparable to or exceeding Lipofectamine 3000 at low Ca2+ concentrations.
- The intrinsic far-red fluorescence of the PD enabled effective tracking of gene delivery.
- The developed platform is biocompatible.
Conclusions:
- The minimalistic, intrinsically fluorescent pseudodendrimer (PD) platform offers efficient and traceable gene delivery.
- Arginine-functionalized PD (TGP 41) forms stable nanocondensates (NCs) with high transfection efficiency.
- This biocompatible PD platform is a versatile tool for nucleic acid theranostics, offering advantages in fluorescence tracking.
