Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Neurogenesis and Regeneration of Nervous Tissue01:15

Neurogenesis and Regeneration of Nervous Tissue

899
In the CNS, neurogenesis, the birth of new neurons from stem cells, is limited to the hippocampus in adults. In other regions of the brain and spinal cord, neurogenesis is almost non-existent due to inhibitory influences from neuroglia, especially oligodendrocytes, and the absence of growth-stimulating cues. The myelin produced by oligodendrocytes in the CNS inhibits neuronal regeneration. Furthermore, astrocytes proliferate rapidly after neuronal damage, forming scar tissue that physically...
899

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gasdermin D-Mediated Release of IL-33 Results in Fetal Brain Developmental Abnormalities During Maternal Colitis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Correction: Cancer cell employs a microenvironmental neural signal trans-activating nucleus-mitochondria coordination to acquire stemness.

Signal transduction and targeted therapy·2025
Same author

Myeloid PD-1 Regulates Astrocyte Development and Leads to Active Behaviours.

Cell proliferation·2025
Same author

Unraveling Neurodevelopment: Synergistic Effects of Intrinsic Genetic Programs and Extrinsic Environmental Cues.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

PD-L1/PD-1 checkpoint pathway regulates astrocyte morphogenesis and myelination during brain development.

Molecular psychiatry·2025
Same author

IFITM2 Modulates Endocytosis Maintaining Neural Stem Cells in Developing Neocortex.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025

Related Experiment Video

Updated: Aug 1, 2025

Assaying Circuit Specific Regulation of Adult Hippocampal Neural Precursor Cells
08:52

Assaying Circuit Specific Regulation of Adult Hippocampal Neural Precursor Cells

Published on: July 24, 2019

6.5K

Ccdc25 regulates neurogenesis during the brain development.

Chong Wang1,2, Jie Qin2,3,4, Jianwei Jiao2,5

  • 1School of Life Sciences, University of Science and Technology of China, Hefei, China.

Developmental Neurobiology
|April 24, 2023
PubMed
Summary

The Ccdc25 protein regulates neural stem cell (NSC) proliferation and differentiation during embryonic brain development. Its function impacts neuronal maturation and is linked to Egr1, influencing neurogenesis.

Keywords:
Ccdc25cerebral cortexneural stem cellneurogenesis

More Related Videos

Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells NPCs
10:47

Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells NPCs

Published on: March 2, 2018

10.1K
Methods for the Modulation and Analysis of NF-κB-dependent Adult Neurogenesis
14:58

Methods for the Modulation and Analysis of NF-κB-dependent Adult Neurogenesis

Published on: February 13, 2014

14.4K

Related Experiment Videos

Last Updated: Aug 1, 2025

Assaying Circuit Specific Regulation of Adult Hippocampal Neural Precursor Cells
08:52

Assaying Circuit Specific Regulation of Adult Hippocampal Neural Precursor Cells

Published on: July 24, 2019

6.5K
Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells NPCs
10:47

Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells NPCs

Published on: March 2, 2018

10.1K
Methods for the Modulation and Analysis of NF-κB-dependent Adult Neurogenesis
14:58

Methods for the Modulation and Analysis of NF-κB-dependent Adult Neurogenesis

Published on: February 13, 2014

14.4K

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cell Biology

Background:

  • Neural stem cell (NSC) proliferation and differentiation are critical for embryonic brain development.
  • Defects in neurogenesis can lead to severe developmental disorders.
  • The role of the Ccdc25 protein in neural systems was previously unclear.

Purpose of the Study:

  • To investigate the function of Ccdc25 in neural stem cells during early embryonic development.
  • To elucidate the molecular mechanisms by which Ccdc25 influences neurogenesis and neuronal maturation.

Main Methods:

  • Utilized shRNA-mediated suppression and overexpression of Ccdc25 in neural stem cells.
  • Assessed NSC proliferation using Ki67 and BrdU markers.
  • Performed RNA sequencing to analyze gene expression changes upon Ccdc25 knockdown.
  • Investigated the role of Egr1 in Ccdc25-mediated neurogenesis.

Main Results:

  • Ccdc25 suppression increased NSC proliferation and inhibited premature terminal mitosis and neuronal differentiation.
  • Ccdc25 overexpression inhibited proliferation and promoted differentiation of NSCs.
  • Ccdc25 knockdown impaired neuronal maturation, reducing branching and axon length.
  • Ccdc25 regulates NSC development through Egr1; Egr1 manipulation mimicked or rescued Ccdc25 knockdown phenotypes.

Conclusions:

  • Ccdc25 plays a significant role in regulating neural stem cell proliferation, differentiation, and neuronal maturation.
  • The Ccdc25-Egr1 axis is a key pathway controlling neurogenesis and brain development.