Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

Plant Cell Wall

Plant Cell Wall

The plant cell wall gives plant cells shape, support, and protection. As a cell matures, its cell wall specializes according to the cell type. For example, the parenchyma cells of leaves possess only a thin, primary cell wall.

Cell Signaling in Plants

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...

Plant Cells and Tissues

Plant Cells and Tissues

Plant tissues are collections of similar cells performing related functions. Different plant tissues will have their own specialized roles and can be combined with other tissues to form organs such as flowers, fruit, stem, and leaves. Two major types of plant tissue include meristematic and permanent tissue.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

CIMatcher: Cross-scale interaction matcher for accurate local feature matching.

Neural networks : the official journal of the International Neural Network Society·2026

Same author

The <i>FHY3</i>/<i>FAR1</i> Gene Family in Plants: Transposase-Derived Transcription Factors as Master Integrators of Light Signaling and Plant Development.

Plants (Basel, Switzerland)·2026

Same author

Transcriptome-Based Identification of <i>AP2/EREBP</i> Genes Regulating Cuticle Formation in Tree Peony 'Bai Wang Shi Zi'.

Plants (Basel, Switzerland)·2026

Same author

Letter to the editor: A comment on statistical power in high-dose radiation group analysis.

The British journal of radiology·2026

Same author

Predicting Frailty Trajectories Using Interpretable Machine Learning Among Older Adults Following Hip Surgery: Prospective Longitudinal Study.

JMIR aging·2026

Same author

Mechanisms underlying green flower formation.

Horticulture research·2026

Search research articles

Related Experiment Video

Updated: Aug 1, 2025

Isolation and Transcriptome Analysis of Plant Cell Types

Isolation and Transcriptome Analysis of Plant Cell Types

Published on: April 7, 2023

Isolation and Transcriptome Analysis of Plant Cell Types.

Jun Zhang¹, Mayra Ahmad¹, Rongrong Xie¹

¹Joint Center for Single Cell Biology, School of Agriculture and Biology, Shanghai Jiao Tong University.

Journal of Visualized Experiments : Jove

|April 24, 2023

Summary

This study presents a fluorescence-activated cell sorting (FACS) protocol for isolating plant cells, enabling single-cell analysis. This method facilitates downstream multi-omics studies, advancing plant science research.

More Related Videos

Fluorescence Activated Cell Sorting of Plant Protoplasts

Fluorescence Activated Cell Sorting of Plant Protoplasts

Published on: February 18, 2010

A Robotic Platform for High-throughput Protoplast Isolation and Transformation

A Robotic Platform for High-throughput Protoplast Isolation and Transformation

Published on: September 27, 2016

Related Experiment Videos

Last Updated: Aug 1, 2025

Isolation and Transcriptome Analysis of Plant Cell Types

Isolation and Transcriptome Analysis of Plant Cell Types

Published on: April 7, 2023

Fluorescence Activated Cell Sorting of Plant Protoplasts

Fluorescence Activated Cell Sorting of Plant Protoplasts

Published on: February 18, 2010

A Robotic Platform for High-throughput Protoplast Isolation and Transformation

A Robotic Platform for High-throughput Protoplast Isolation and Transformation

Published on: September 27, 2016

Area of Science:

Plant Biology
Cellular Biology
Genomics

Background:

Cellular heterogeneity is crucial for development and environmental responses in multicellular organisms.
Single-cell resolution studies are vital but challenging in plants due to cell walls.
Next-generation sequencing (NGS) techniques require efficient single-cell isolation.

Purpose of the Study:

To develop a robust fluorescence-activated cell sorting (FACS) protocol for single plant cell isolation.
To enable downstream multi-omics analyses, including transcriptomics, at the single-cell level.
To overcome limitations in plant single-cell isolation for broader research applications.

Main Methods:

Developed a fluorescence-activated cell sorting (FACS) protocol for plant cell isolation.
Utilized Arabidopsis root fluorescent marker lines for cell-type specific sorting.
Implemented Smart-seq2 for downstream transcriptome analysis of isolated cells.

Main Results:

Successfully isolated specific Arabidopsis root cell types, including xylem-pole pericycle, lateral root initials, lateral root cap, cortex, and endodermal cells.
Demonstrated the effectiveness of the FACS method for downstream transcriptome analysis.
Validated the protocol's adaptability for various cell types and plant species.

Conclusions:

The developed FACS protocol provides a robust method for single plant cell isolation.
This technique supports diverse downstream analyses, significantly advancing plant single-cell research.
The protocol has broad applicability across plant science, facilitating studies on cellular heterogeneity and development.