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G2 arrest, binucleation, and single-parameter DNA flow cytometric analysis.

X Ronot, C Hecquet, S Larno

    Cytometry
    |May 1, 1986
    PubMed
    Summary
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    Flow cytometry can misinterpret cell cycle arrest due to drug effects on cytokinesis. Binucleate cells induced by sodium butyrate and D-penicillamine can appear as G2 arrest, highlighting interpretation challenges.

    Area of Science:

    • Cell Biology
    • Pharmacology
    • Cytometry

    Background:

    • Flow cytometry is crucial for analyzing cell cycle progression.
    • Pharmacological agents can significantly impact cell cycle dynamics.
    • Accurate interpretation of flow cytometry data is essential for understanding drug mechanisms.

    Purpose of the Study:

    • To investigate the effects of pharmacological agents on cell cycle progression using flow cytometry.
    • To compare the impact of sodium butyrate, SOAZ, and D-penicillamine on cell cycle distribution.
    • To identify potential artifacts in flow cytometry analysis caused by drug-induced cytokinesis perturbation.

    Main Methods:

    • Comparative analysis of G2 fraction perturbations in articular chondrocytes and HeLa cells.
    • Treatment with sodium butyrate, an antineoplastic agent (SOAZ), and an antirheumatic drug (D-penicillamine).

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  • DNA flow cytometric analysis to assess cell cycle distribution.

    • Main Results:

    • While DNA flow cytometry often detects G2 arrest, the agents had different mechanisms of action.
    • Sodium butyrate and D-penicillamine increased binucleate cells by perturbing cytokinesis.
    • Distinguishing G2 cells from binucleate G0/1 cells was challenging due to similar fluorescence intensity, indicating a limitation in detecting binucleate cells.

    Conclusions:

    • DNA flow cytometry measurements must be interpreted cautiously when cells are exposed to agents affecting cytokinesis.
    • Cytokinesis perturbation can lead to artifacts in flow cytometry histograms, mimicking G2 arrest.
    • Rapid single-cell cycle analysis is recommended for studying pharmacological interactions to avoid misinterpretation.