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Related Experiment Videos

Cell-cooling in flow cytometry by Peltier elements.

C Göttlinger, K L Meyer, W Weichel

    Cytometry
    |May 1, 1986
    PubMed
    Summary
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    A novel Peltier effect cooling device maintains cell suspensions at 2-5°C for flow cytometry. This easy-to-build prototype effectively cools small fluid volumes during long cell sorting procedures.

    Area of Science:

    • Biotechnology
    • Biophysics

    Background:

    • Maintaining sample integrity during extended flow cytometry is crucial.
    • Existing methods for cooling cell suspensions can be cumbersome or inefficient.

    Purpose of the Study:

    • To develop and evaluate a Peltier effect-based cooling device for cell suspensions.
    • To assess the device's capability in maintaining low temperatures during flow cytometry.

    Main Methods:

    • Construction of a prototype cooling device utilizing the Peltier effect.
    • Testing the device's performance with up to 3 ml of cell suspension.
    • Monitoring temperature to ensure a stable 2-5°C range.

    Main Results:

    • The prototype successfully maintained cell suspensions at 2-5°C.

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  • Effective cooling was achieved for volumes up to 3 ml.
  • The device demonstrated ease of construction and handling.

    • Conclusions:

    • Peltier element-based cooling is a viable method for temperature control of small fluid volumes.
    • The developed cooling device is suitable for long-duration cell sorting in flow cytometry.
    • This technology offers a simple and effective solution for preserving cell viability.