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Converting Acellular Dermal Matrix into On-Demand Versatile Skin Scaffolds by a Balanceable Crosslinking Approach for
Yining Chen1,2, Ying Zhang3, Qi Wang3
1Key Laboratory of Leather Chemistry and Engineering (Sichuan University), Ministry of Education, Chengdu 610065, China.
Biomacromolecules
|April 24, 2023
Summary
This study introduces an innovative crosslinking method using oxidized 2-hydroxypropyltrimethyl ammonium chloride chitosan (OHTCC) to enhance acellular dermal matrix (ADM) skin scaffolds. The modified scaffolds offer improved antibacterial and mechanical properties for effective infected wound healing.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Wound Healing
Background:
- Acellular dermal matrix (ADM) is a common skin graft substitute but lacks sufficient antibacterial and mechanical properties.
- Existing limitations hinder the clinical application of ADM in treating infected wounds.
Purpose of the Study:
- To develop a versatile skin scaffold using a novel crosslinking approach for integrated infected wound therapy.
- To enhance the functionality of ADM by incorporating oxidized 2-hydroxypropyltrimethyl ammonium chloride chitosan (OHTCC).
Main Methods:
- A balanceable crosslinking strategy was employed using OHTCC with varying oxidation degrees to modify ADM.
- Physicochemical properties, antibacterial efficacy, and cytocompatibility of the OHTCC-ADM scaffolds were evaluated.
- In vitro and in vivo studies assessed the therapeutic potential for infected wound healing.
Main Results:
- OHTCC-ADM scaffolds with an optimal oxidation degree (~13%) exhibited balanced physiochemical properties, enhanced antibacterial activity, and good cytocompatibility.
- The scaffolds demonstrated adjustable mechanical properties, degradability, and thermal stability.
- In vitro/in vivo studies confirmed broad-spectrum antibacterial performance, reduced inflammation, and promoted tissue regeneration.
Conclusions:
- The developed OHTCC crosslinking method offers a facile approach to create versatile ADM-based skin scaffolds.
- These enhanced scaffolds show significant potential for integrated therapy of infected wounds, improving healing outcomes.
